A novel Nb2C MXene based aptasensor for rapid and sensitive multi-mode detection of AFB1

适体 荧光 生物传感器 材料科学 纳米技术 量子力学 遗传学 物理 生物
作者
Yiqian Kong,Zongyi Li,Lili Zhang,Juncheng Song,Qi Liu,Yinghua Zhu,Na Li,Lili Song,Xiangyang Li
出处
期刊:Biosensors and Bioelectronics [Elsevier BV]
卷期号:242: 115725-115725 被引量:27
标识
DOI:10.1016/j.bios.2023.115725
摘要

Rapid and accurate on-site detection of aflatoxin B1 (AFB1) is of great significance for ensuring food safety. This work developed a dual mode aptasensor and a dual channel artificial neural network (ANN) intelligent sensor detection platform for simple and convenient quantitative detection of AFB1 in food. This sensor was prepared by encoding manganese ion (Mn2+) mediated surface concave niobium carbide MXene nanomaterials (Nb2C-MNs) using fluorescent group labeled aptamers (ssDNA-FAM). Mn2+-mediated Nb2C-MNs exhibited better peroxidase-like and fluorescence quenching properties. Moreover, ssDNA-FAM as a fluorescent probe for the sensor also significantly enhanced the enzyme activity of Nb2C-MNs. When AFB1 existed, ssDNA-FAM preferentially bonded to AFB1, resulting in fluorescence signal recovery and colorimetric signal weakening. Consequently, the multimodal biosensor could achieve fluorescence/colorimetric detection without the need for material and reagent replacement. In on-site detection, both ratio fluorescence and colorimetric signals could be collected using smartphones and analyzed and modeled on the developed ANN platform, achieving visual intelligent sensing. This multimodal biosensor had a detection line as low as 0.0950 ng/mL under optimal conditions, and also had the advantages of simple operation, fast and sensitive, and high specificity, which can meet the real-time on-site detection needs of AFB1 in remote areas.
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