已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

miR-153 Targets Autophagy and Apoptosis Caspase Family to Explore the Molecular Mechanism of Polystyrene Nanoparticles Intervening in Thoracoabdominal Aortic Aneurysm

细胞凋亡 转染 癌症研究 自噬 动脉瘤 主动脉瘤 转移 小RNA 化学 动脉瘤 医学 基因 内科学 外科 癌症 生物化学
作者
Linru Wang,Pinyi Wang,Yunfei Cui,Wenxian Hu
出处
期刊:Journal of Biomedical Nanotechnology [American Scientific Publishers]
卷期号:19 (7): 1199-1205
标识
DOI:10.1166/jbn.2023.3638
摘要

This study investigates whether miR-153 regulates the expression of autophagy and apoptosis-related gene Caspase3, and analyzes the role of polystyrene nanoparticles in thoracoabdominal aortic aneurysm cells. Surgically resected thoracoabdominal aortic aneurysm tissue samples were included as the observation group and the control group included 2 cm tissues from the side of thoracoabdominal aortic aneurysm. The mRNA levels of miR-153 and Caspase3 were assessed by reverse transcription polymerase chain reaction (RT-PCR) and cell proliferation, apoptosis, invasion, and migration were evaluated. Tissue microarray and RT-PCR showed that miR-153 was expressed in tissues and it displayed a targeted relationship with Caspase3. Transwell results showed that transfection of miR-153-targeted Caspase3 nanoparticles significantly reduced the infiltration of thoracic and aortic aneurysm. After si-RNA interfered with the expression of miR-153, the proliferation rate of thoracoabdominal aortic aneurysms in the miR-153-targeted Caspase3-directed nanoparticle group was significantly reduced. On the contrary, when miR-153 was overexpressed, the apoptosis was significantly reduced. Transfection of si-miR-153-targeting Caspase3 nanoparticles significantly reduced the invasion and metastasis ability of thoracoabdominal aortic aneurysm cells and the migration ability was increased after reversing miR-153 expression. The decreased expression of miR-153 is related to the increased expression of Caspase3 and enhanced invasion and metastasis of thoracoabdominal aortic aneurysms. Overexpression of miR-153 can inhibit the expression of Caspase3 and targeted nanoparticles can effectively enhance cell activities.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
2秒前
2秒前
mouxq发布了新的文献求助10
5秒前
Jumping完成签到 ,获得积分10
5秒前
cvvl2发布了新的文献求助10
5秒前
科研通AI2S应助过儿采纳,获得10
8秒前
9秒前
10秒前
独孤阳光发布了新的文献求助10
11秒前
12秒前
chandangfo完成签到,获得积分10
15秒前
gxy发布了新的文献求助10
15秒前
19秒前
清爽的雨竹完成签到 ,获得积分10
19秒前
21秒前
21秒前
21秒前
好汉发布了新的文献求助10
24秒前
24秒前
tian完成签到 ,获得积分10
25秒前
研友_燚孟为马完成签到,获得积分20
25秒前
Akim应助过儿采纳,获得30
25秒前
kk完成签到,获得积分10
29秒前
xixi发布了新的文献求助30
31秒前
31秒前
Ava应助88采纳,获得10
34秒前
39秒前
好汉完成签到,获得积分10
40秒前
毛豆应助苦木采纳,获得10
40秒前
韦自中完成签到,获得积分10
41秒前
叶落孤城完成签到 ,获得积分10
42秒前
乐乐应助过儿采纳,获得10
42秒前
42秒前
43秒前
43秒前
HHYYAA发布了新的文献求助10
44秒前
45秒前
科研通AI2S应助77采纳,获得10
46秒前
周淡念完成签到,获得积分20
46秒前
高分求助中
Востребованный временем 2500
Hopemont Capacity Assessment Interview manual and scoring guide 1000
Injection and Compression Molding Fundamentals 1000
Classics in Total Synthesis IV: New Targets, Strategies, Methods 1000
Mantids of the euro-mediterranean area 600
The Oxford Handbook of Educational Psychology 600
Mantodea of the World: Species Catalog Andrew M 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 内科学 物理 纳米技术 计算机科学 基因 遗传学 化学工程 复合材料 免疫学 物理化学 细胞生物学 催化作用 病理
热门帖子
关注 科研通微信公众号,转发送积分 3422705
求助须知:如何正确求助?哪些是违规求助? 3023064
关于积分的说明 8903264
捐赠科研通 2710478
什么是DOI,文献DOI怎么找? 1486502
科研通“疑难数据库(出版商)”最低求助积分说明 687061
邀请新用户注册赠送积分活动 682299