已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Integrin‐Linked Kinase in the Development of Gastric Tumors Induced by Helicobacter pylori: Regulation and Prevention Potential

整合素连接激酶 CTGF公司 癌症研究 激酶 基因敲除 河马信号通路 癌变 日历年61 化学 幽门螺杆菌 分子生物学 癌症 生物 蛋白激酶A 细胞凋亡 医学 内科学 细胞生物学 生长因子 细胞周期蛋白依赖激酶2 受体 生物化学
作者
Boqing Li,Jing He,Ruiqing Zhang,Sisi Liu,Xiaolin Zhang,Zhiqin Li,Chunlei Ma,W. Wang,Yingzi Cui,Ying Zhang
出处
期刊:Helicobacter [Wiley]
卷期号:29 (4)
标识
DOI:10.1111/hel.13109
摘要

ABSTRACT Background Integrin‐linked kinase (ILK) is crucial in solid tumors by regulating the Hippo‐Yes‐associated protein 1 (YAP) pathway. This study aimed to uncover how Helicobacter pylori influences ILK levels and its role in regulating YAP during H. pylori ‐induced gastric cancer. Materials and Methods GES‐1 cells with stable Ilk knockdown and overexpression and a mouse carcinogenesis model for H. pylori infection were constructed. And ILK, the phosphorylated mammalian STE20‐like protein kinase 1 (MST1), large tumor suppressor 1 (LATS1; S909, T1079), and YAP (S109, S127) were detected in cells, and mice by western blotting, as well as fluorescence intensity of YAP were assayed by immunofluorescence. YAP downstream genes Igfbp4 and Ctgf , the pathological changes and tumor necrosis factor alpha (TNF‐α), interleukin‐6 (IL‐6), interleukin‐1beta (IL‐1β), and nitric oxide (NO) levels in mice gastric tissues were detected by real‐time PCR, H&E, and ELISA assays. Results In this study, stable Ilk knockdown cells exhibited significantly higher phosphorylated levels of MST1, LATS1, and YAP, as well as increased YAP in the nuclei of GES‐1 cells. Conversely, cells with Ilk overexpression showed opposite results. H. pylori infection led to decreased ILK levels in gastric epithelial cells but increased ILK levels in gastric cancer cell lines (MGC803, SGC7901) and gastric cancer tissues in mice. Treatment with the ILK inhibitor OST‐T315 elevated the phosphorylated MST, LATS1, and YAP levels, and inhibited the mRNA levels of Igfbp4 and Ctgf at 44, 48 week‐aged mice. OST‐T315 also reduced the release of TNF‐α, IL‐6, IL‐1β, and NO, as well as the progression of gastric cancer caused by H. pylori and N ‐Nitroso‐ N ‐methylurea (NMU) treatment. Conclusion Upon initiation of gastric tumorigenesis signals, H. pylori increases ILK levels and suppresses Hippo signaling, thereby promoting YAP activation and gastric cancer progression. ILK can serve as a potential prevention target to impede H. pylori ‐induced gastric cancer.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
田様应助jz采纳,获得10
1秒前
英俊的铭应助panda采纳,获得10
1秒前
2秒前
4秒前
6秒前
思嗡完成签到 ,获得积分10
6秒前
StoneT完成签到,获得积分20
7秒前
chun发布了新的文献求助10
7秒前
灵巧的含海完成签到,获得积分10
8秒前
Gao小白完成签到,获得积分10
8秒前
孤独树叶发布了新的文献求助10
9秒前
sherry0019发布了新的文献求助10
10秒前
南昌黑人发布了新的文献求助10
12秒前
13秒前
yowgo完成签到,获得积分10
13秒前
13秒前
zqy1111发布了新的文献求助10
16秒前
cc发布了新的文献求助10
18秒前
18秒前
23秒前
25秒前
25秒前
nn发布了新的文献求助10
26秒前
Akim应助panda采纳,获得10
27秒前
小蘑菇应助曦月采纳,获得10
27秒前
充电宝应助hhgcc采纳,获得10
27秒前
29秒前
30秒前
32秒前
33秒前
orixero应助等待夏旋采纳,获得10
34秒前
单身的溪流完成签到,获得积分10
35秒前
方赫然应助SUO采纳,获得10
36秒前
38秒前
谭凯文发布了新的文献求助10
38秒前
39秒前
曦月发布了新的文献求助10
39秒前
科研通AI2S应助nano采纳,获得10
40秒前
41秒前
jz发布了新的文献求助10
43秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 830
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3248577
求助须知:如何正确求助?哪些是违规求助? 2892029
关于积分的说明 8269412
捐赠科研通 2560089
什么是DOI,文献DOI怎么找? 1388851
科研通“疑难数据库(出版商)”最低求助积分说明 650913
邀请新用户注册赠送积分活动 627798