CHAPTER 3. Preparation of Cultured Cell-derived Decellularized Matrix (dECM) – Factors Influencing dECM Formation and Its Ability

There are two types of decellularized extracellular matrix (dECM): tissue- or organ-derived dECM and cultured cell-derived dECM. Although it is difficult to prepare cultured cell-derived dECM, which is completely the same as the native extracellular matrix (ECM), it has several advantages over tissue- or organ-derived dECM. Thus, cultured cell-derived dECM has been developed for various purposes. This chapter summarizes the points that need to be considered for the preparation and assessment of cell-derived dECM, after a brief summary of the ECM formation processes. The applications of cultured cell-derived dECM are then briefly reviewed. Finally, the problems to be solved in the future for the progress of cultured cell-derived dECM technology are described.