生物
微生物学
减毒疫苗
病菌
毒力
免疫
免疫系统
突变体
半数致死剂量
感染剂量
致死剂量
病毒学
免疫学
毒性
基因
生物化学
病毒
毒理
有机化学
化学
作者
Haoda Ye,Zhijin Xu,Zhen Tao,Weiye Li,Yanyan Li,Amin Yang,Wei Wang,Xiaolong Yin,Xiaojun Yan
出处
期刊:Aquaculture
[Elsevier]
日期:2021-01-01
卷期号:531: 735976-735976
被引量:16
标识
DOI:10.1016/j.aquaculture.2020.735976
摘要
Pseudomonas plecoglossicida is a Gram-negative fish pathogen that affects the large yellow croaker (LYC; Larimichthys crocea) and several other economically important fish species. The bacterium can cause systemic infections in LYC, resulting in visceral granulomas disease (VGD) with high mortality in cultured populations. Despite the severity of this disease, the LYC industry lacks an effective vaccine for VGD. We have constructed a highly attenuated P. plecoglossicida mutant by deleting a virulence gene (tssD-1) from the wild-type (WT) strain XSDHY-P. We then investigated the safety and efficacy of the mutant strain ΔtssD-1 in LYC juveniles as a live attenuated vaccine candidate. LYCs were vaccinated with ΔtssD-1 by two intraperitoneal injections per fish, and then challenged with P. plecoglossicida XSDHY-P by the immersion route. Vaccination of ΔtssD-1 induced a significantly higher level of P. plecoglossicida-specific serum IgM and increased expression of CD8α and MHCIα, and resulted in a relative percentage survival of 86.3%. The safety of ΔtssD-1 was demonstrated because no mortality or clinical signs were found in a LYC group injected with a high dose (1.8 × 106 CFU per fish) of ΔtssD-1. This is the first report of a defined live attenuated strain providing protective immunity in LYC against P. plecoglossicida.
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