隐孢子虫
放大器
微小隐孢子虫
生物
套式聚合酶链反应
聚合酶链反应
微生物学
病毒学
遗传学
基因
粪便
作者
Guy Robinson,Kristin Elwin,Rachel M. Chalmers
出处
期刊:Methods in molecular biology
日期:2019-08-27
卷期号:: 11-22
被引量:21
标识
DOI:10.1007/978-1-4939-9748-0_2
摘要
Molecular diagnostic assays for Cryptosporidium are usually based on PCR and may detect the entire genus or target specified species. Of the ~40 species, fewer than half have been reported from humans, and most human cases of cryptosporidiosis are caused by Cryptosporidium parvum or Cryptosporidium hominis. Here we describe a nested PCR for the detection of all Cryptosporidium spp. that can then be differentiated by sequencing the PCR amplicons, and a duplex, real-time PCR for the simultaneous detection and differentiation of C. parvum and C. hominis.
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