Microbial interspecific interaction and nitrogen metabolism pathway for the treatment of municipal wastewater by iron carbon based constructed wetland

In order to explore the pollutant removal performance and interspecific interaction in constructed wetland (CW) with Fe0-C filler, constructed wetland with Fe0-C filler (CW-Fe) and with ceramsite filler (CW-C) were set up. Besides, the nutrients removal and interspecific interaction were analyzed, and the results showed that total nitrogen (TN) removal efficiency of CW-Fe system without carbon source was lower than that in CW-C system though CW-Fe system could convert macro-molecular organic matter into micro-molecular organic matter. However, ammonia nitrogen (NH4+-N) increase was observed in CW-Fe system with better total phosphorus (TP) removal performance. High-throughput sequencing showed that the microbial richness and abundance of Bacteroides, Firmicutes, Chlorofeli and Actinobacteria in the CW with Fe0-C filler was significantly higher than with ceramsite filler. The interaction between two CWs was significantly different, and the functional enzymes abundance of nitrate nitrogen (NO3--N) to NH4+-N transformation in CW-Fe system significantly increased.