微通道
材料科学
电泳
电场
微流控
层流
生物分子
色谱法
毛细管电泳
纳米技术
化学
量子力学
热力学
物理
作者
Hongli Liu,Fengyun Li,Lizhi Zhao,Lei Zhao,Ping Sun,Jing Wu,Xiayan Wang,Qiaosheng Pu
标识
DOI:10.1021/acsami.9b22878
摘要
Electrophoretic separation in short microchannels is a promising way for rapid analysis of biomolecules, but the pressurized laminar flow may compromise the separation efficiency. In this work, through an electric field, instant formation and removal of a solid chitosan/β-glycerol phosphate (CS/β-GP) hydrogel within microchannels of microchips were realized. In a typical cross-type microchip, the CS/β-GP hydrogel was precisely formed in the separation microchannel within 15 s of the application of a voltage of 2000 V. Highly efficient separation of peptides and proteins was achieved, and theoretical plate numbers of 0.6 to 1.5 × 106/m were attained for proteins in 120 s. The used hydrogel could be swiftly removed also with an electric field, and the whole procedure was achieved on a standard microchip electrophoresis device with no extra accessory or special operation required.
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