Pore size assessment during corneal endothelial cell permeabilization by femtosecond laser-activated carbon nanoparticles

钙黄绿素 右旋糖酐 活力测定 生物物理学 流式细胞术 材料科学 飞秒 化学 纳米技术 细胞 激光器 色谱法 分子生物学 生物化学 生物 光学 物理
作者
Clotilde Jumelle,Cyril Mauclair,J. Houzet,Bernard Kress,Ziyi He,S PISELLI,Chantal Perrache,Philippe Gain,Gilles Thuret
出处
期刊:Acta Ophthalmologica [Wiley]
卷期号:93: n/a-n/a
标识
DOI:10.1111/j.1755-3768.2015.0595
摘要

Purpose Therapeutic molecules delivery represents a promising solution to maintain human corneal endothelial cells (HCECs) viability, but transport across cell membrane must be facilitated. A new delivery method consists in ephemerally permeabilizing cell membranes using a photo-acoustic reaction produced by carbon nanoparticles (CNPs) and femtosecond laser (FsL). The aim of this work is to investigate the size of pores formed at cell membrane by this technique. Methods To induce cell permeabilization, HCECs (B4G12 cell line) were put in contact with CNPs and irradiated with a 500 µm diameter Ti:Sa FsL focalized spot. Four sizes of fluorescent reporter molecules were delivered into HCECs to investigate pore sizes: calcein (1.2 nm), FITC-Dextran 4 kDa (2.8 nm) and FITC-Dextran 70 kDa (12 nm) and FITC-Dextran 2MDa (50 nm). Uptake of each molecule was assessed by flow cytometry immediately after irradiation. Results The delivery rate was dependent of their size. Calcein was delivered in 56 ± 8% of HCECs, FITC-Dextran 4 kDa in 42 ± 4%, FITC-Dextran 70 kDa in 22 ± 3% and finally FITC-Dextran 2MDa in 13 ± 2%, suggesting that a large number of pores in the size ranging from 1.2 to 2.8 nm were formed. However, 12 nm and larger pores were almost half more infrequent. Conclusions Pore sizes formed at cell membrane by the technique of cell permeabilization by FsL activated CNPs were investigated for the first time. This innovative non-viral method is characterized by pore sizes large enough for the efficient delivery of small, medium and big therapeutic molecules on HCECs. GRANT: Fondation des Aveugles de France, Fondation de l'Avenir, Fondation Visaudio (ET1-638).

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
张演基发布了新的文献求助20
2秒前
3秒前
5秒前
6秒前
6秒前
6秒前
lsx完成签到,获得积分10
7秒前
传奇3应助yaya采纳,获得10
8秒前
9秒前
林海发布了新的文献求助10
9秒前
幸福大白发布了新的文献求助10
9秒前
10秒前
无花果应助沐言采纳,获得10
10秒前
10秒前
我是站长才怪应助lfl采纳,获得10
11秒前
march发布了新的文献求助30
11秒前
萧水白应助等待的莞采纳,获得10
12秒前
友好芾发布了新的文献求助10
12秒前
张演基完成签到,获得积分10
12秒前
研友_VZG7GZ应助李子采纳,获得10
13秒前
13秒前
顾矜应助无心的初珍采纳,获得10
14秒前
wax发布了新的文献求助200
14秒前
乐观帅哥完成签到,获得积分10
15秒前
15秒前
15秒前
洛洛发布了新的文献求助10
15秒前
creep完成签到,获得积分10
15秒前
16秒前
16秒前
罗伯特发布了新的文献求助10
16秒前
17秒前
18秒前
18秒前
19秒前
20秒前
21秒前
科研通AI2S应助lfl采纳,获得10
21秒前
碧蓝板栗发布了新的文献求助20
23秒前
文静三颜发布了新的文献求助10
25秒前
高分求助中
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger Heßler, Claudia, Rud 1000
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 1000
Natural History of Mantodea 螳螂的自然史 1000
A Photographic Guide to Mantis of China 常见螳螂野外识别手册 800
Autoregulatory progressive resistance exercise: linear versus a velocity-based flexible model 500
Spatial Political Economy: Uneven Development and the Production of Nature in Chile 400
Research on managing groups and teams 300
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3330040
求助须知:如何正确求助?哪些是违规求助? 2959654
关于积分的说明 8596227
捐赠科研通 2638022
什么是DOI,文献DOI怎么找? 1444115
科研通“疑难数据库(出版商)”最低求助积分说明 668935
邀请新用户注册赠送积分活动 656517