色谱法
化学
电喷雾电离
醋酸铵
绿原酸
选择性反应监测
萃取(化学)
串联质谱法
咖啡酸
高效液相色谱法
质谱法
乙酸乙酯
分析物
药代动力学
液相色谱-质谱法
检出限
内科学
抗氧化剂
医学
生物化学
作者
Chunli Gan,Lu Liu,Yan Du,Liqian Wang,Mingjie Gao,Lijun Wu,Chunjuan Yang
标识
DOI:10.1002/jssc.201600051
摘要
A rapid and sensitive assay based on ultra-high performance liquid chromatography with electrospray ionization tandem mass spectrometry was established and validated for the simultaneous determination of cichoric acid, chlorogenic acid, quinic acid, and caffeic acid in rat plasma after oral administration of Echinacea purpurea extract using butylparaben as the internal standard. Samples were pretreated by liquid-liquid extraction with ethyl acetate. The separations for analytes were performed on an ACQUITY UPLC HSS C18 column (1.8 μm 2.1 × 100 mm) using a gradient elution program with acetonitrile/10 mM ammonium acetate (pH 5.6) at a flow rate of 0.3 mL/min. The analytes were detected in multiple reaction monitoring mode with negative electrospray ionization. The lower limit of quantification of each analyte was not higher than 10.85 ng/mL. The relative standard deviation of the intraday and interday precisions was less than 14.69%. The relative errors of accuracies were in the range of -13.80 to 14.91%. The mean recoveries for extraction recovery and matrix effect were higher than 80.79 and 89.98%, respectively. The method validation results demonstrated that the proposed method was sensitive, specific, and reliable, which was successfully applied to the pharmacokinetic study of four components after oral administration of Echinacea purpurea extract.
科研通智能强力驱动
Strongly Powered by AbleSci AI