Hollow and Porous Fe3C-NC Nanoballoons Nanozymes for Cancer Cell H2O2 Detection

In this work, we reported a new template-induced strategy for the controlled synthesis of hollow and porous Fe3C-NC nanoballoons (Fe3C-NC-NBs) with intrinsic peroxidase-like activity. The resulting Fe3C-NC-NBs nanozymes with a large specific surface area, rich porous structures, a high N and Fe content exhibit excellent performance including large linear range and low detection limit, good reproducibility and selectivity for detecting H2O2 both in buffer solution and released from Hela cells via a fluorescence assay. This work provides a new strategy for controlled construction of novel M-NC hybrid and expand the applications of Fe-NC nanomaterials as high-efficiency nanozymes in biological detection.