生物传感器
葡萄糖氧化酶
检出限
微流控
化学
色谱法
免疫磁选
催化作用
磁性纳米粒子
纳米颗粒
材料科学
纳米技术
生物化学
作者
Yingjia Liu,Dong Jiang,Siyuan Wang,He Zhang,Xue Li,Yanbin Li,Ming Liao,Jianhan Lin
标识
DOI:10.1016/j.cclet.2021.10.064
摘要
Rapid screening of foodborne pathogens is of great significance to ensure food safety. A microfluidic biosensor based on immunomagnetic separation, enzyme catalysis and electrochemical impedance analysis was developed for rapid and sensitive detection of S. typhimurium. First, the bacterial sample, the magnetic nanoparticles (MNPs) modified with capture antibodies, and the enzymatic probes modified with detection antibodies and glucose oxidase (GOx) were simultaneously injected into the microfluidic chip, followed by mixing and incubation to form MNP-bacteria-probe sandwich complexes. Then, glucose with high impedance was injected into the chip and catalyzed by the GOx on the complexes into hydrogen peroxide with high impedance and gluconic acid with low impedance, which was finally measured using the low-cost interdigitated microelectrode and the electrochemical impedance analyzer to determine the target bacteria. Under the optimal conditions, this biosensor could quantitatively detect S. typhimurium at the concentrations from 1.6 × 102 CFU/mL to 1.6 × 106 CFU/mL in 1 h with the low detection limit of 73 CFU/mL. Besides, this biosensor was demonstrated with good feasibility for practical applications by detecting the S. typhimurium spiked chicken meat samples.
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