RNA聚合酶Ⅱ
抄写(语言学)
转录因子II F
细胞生物学
核糖核酸
分子生物学
RNA聚合酶
化学
生物
生物化学
发起人
基因
基因表达
语言学
哲学
作者
Isaac Fianu,Ying Chen,Christian Dienemann,Olexandr Dybkov,A. Linden,Henning Urlaub,Patrick Cramer
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2021-11-12
卷期号:374 (6569): 883-887
被引量:97
标识
DOI:10.1126/science.abk0154
摘要
Integrator and protein phosphatase 2A (PP2A) form a complex that dephosphorylates paused RNA polymerase II (Pol II), cleaves the nascent RNA, and terminates transcription. We report the structure of the pretermination complex containing the human Integrator-PP2A complex bound to paused Pol II. Integrator binds Pol II and the pausing factors DSIF and NELF to exclude binding of the elongation factors SPT6 and PAF1 complex. Integrator also binds the C-terminal domain of Pol II and positions PP2A to counteract Pol II phosphorylation and elongation. The Integrator endonuclease docks to the RNA exit site and opens to cleave nascent RNA about 20 nucleotides from the Pol II active site. Integrator does not bind the DNA clamps formed by Pol II and DSIF, enabling release of DNA and transcription termination.
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