生物发光
体内
正电子发射断层摄影术
临床前影像学
葡萄糖摄取
代谢物
分子成像
碳水化合物代谢
化学
生物化学
生物
生物物理学
内分泌学
胰岛素
生物技术
神经科学
作者
Tamara Maric,Georgy Mikhaylov,Pavlo Khodakivskyi,Arkadiy Bazhin,Riccardo Sinisi,Nicolas Guex,Aleksey Yevtodiyenko,Anthony E. Jones,Vishaka Muhunthan,Gihad Abdelhady,David B. Shackelford,Elena A. Dubikovskaya
出处
期刊:Nature Methods
[Springer Nature]
日期:2019-05-13
卷期号:16 (6): 526-532
被引量:56
标识
DOI:10.1038/s41592-019-0421-z
摘要
Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used 18F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development. A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d-glucose absorption both in vitro and in vivo.
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