烟草
免疫沉淀
根癌农杆菌
细胞生物学
生物
表位
农业渗透
体内
生物化学
基因
遗传学
抗体
转基因
作者
Alfonso Muñoz,M. Mar Castellano
出处
期刊:Methods in molecular biology
日期:2018-01-01
卷期号:: 279-287
被引量:20
标识
DOI:10.1007/978-1-4939-7871-7_19
摘要
Protein-protein interactions discovered by yeast two-hybrid systems must be confirmed in vivo in a homologous system. In the case of plants, one of the easiest and fastest methods to validate protein interactions in vivo is the transient expression of the proteins in Nicotiana benthamiana leaves followed by coimmunoprecipitation. This method consists of the following steps: growth of the appropriate Agrobacterium tumefaciens cultures, preparation of the infiltration mixtures, infiltration into N. benthamiana leaves, protein extraction and immunoprecipitation. The utilization of epitope tags to immunoprecipitate and detect the proteins of interest is very useful in this procedure. In this chapter we describe a standard protocol to coimmunoprecipitate proteins expressed in N. benthamiana leaves.
科研通智能强力驱动
Strongly Powered by AbleSci AI