寡核苷酸
过程(计算)
计算机科学
寡核苷酸合成
磷酰胺
生化工程
风险分析(工程)
数据科学
比例(比率)
计算生物学
纳米技术
工程类
生物
医学
地理
材料科学
操作系统
DNA
地图学
遗传学
出处
期刊:The Royal Society of Chemistry eBooks
[The Royal Society of Chemistry]
日期:2019-02-08
卷期号:: 453-473
被引量:9
标识
DOI:10.1039/9781788015714-00453
摘要
An increasing number of therapeutic oligonucleotides are entering human clinical trials, leading to multiple marketed drugs. Thus, large-scale automated synthesis of these products has become a high priority for process chemists. This chapter summarizes the advances in the large-scale solid-support synthesis of oligonucleotides using conventional phosphoramidite chemistry. An overview of the most prevalent modifications currently utilized for the assembly of modified oligonucleotides along with a four-step automated process is presented. A brief description of the post-synthesis processes is also included with protocols for characterization of drug substance. A glimpse of possible future technologies that may tackle the economic and ecological challenges of the 21st century when oligonucleotides will be required in metric-tonne quantity is also discussed.
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