Application progress of immobilized biomembrane in the discovery of active compounds of natural products

Natural products (NP) are an important source of bioactive compounds. Considering their complex matrix effects, the development of suitable methodologies for the quick identification and analysis of active substances in NPs played a significant role in controlling their quality and discovering new drugs. In recent years, the technology of immobilized biomembrane has attracted increasing attention, due to its advantages such as multitarget efficiency, accuracy, and/or time-saving compared with traditional activity-guided separation and ligand fishing methods. This article provides a systematic review of the latest advances in screening technologies based on biomembrane in the field of NPs. It includes detailed discussions on these technologies, including cell membrane chromatography, artificial membrane chromatography, cell membrane fishing, living cell fishing methods, and their applications in screening various active molecules from NPs. Their limitations and future development prospects are further discussed.