Mouse Liver B Cells Phagocytose <i>Streptococcus pneumoniae</i> and Initiate Immune Responses against Their Antigens

生物 免疫系统 体细胞突变 脾脏 肺炎链球菌 B-1电池 微生物学 吞噬作用 免疫球蛋白类转换 白细胞介素12 抗原 免疫学 B细胞 抗体
作者
Masahiro Nakashima,Manabu Kinoshita,Hiroyuki Nakashima,Azusa Kato,Kazuma Mori,Kazuki Koiwai,Nariyoshi Shinomiya,Shuhji Seki
出处
期刊:Journal of Immunology [The American Association of Immunologists]
卷期号:209 (1): 26-37
标识
DOI:10.4049/jimmunol.2100520
摘要

Recent studies have revealed that mammalian B cells ingest particulate Ags, such as bacteria, although little is known about the effect of this function on acquired immunity. We investigated the role of bacterium-phagocytosing B cells in acquired host immune responses. Cultured mouse liver B cells substantially phagocytosed serum-opsonized Streptococcus pneumoniae and produced IgM. On adoptive transfer of liver B cells that phagocytose S. pneumoniae labeled with pHrodo Red succinimidyl ester, recipient mice showed elevated plasma levels of IgG specific for bacterial Ags. In particular, the levels of IgG2a and IgG2b specific for pneumococcal surface protein A, as well as IgG3 for pneumococcal polysaccharide, were markedly increased compared with total IgG specific for each Ag. When phagocytic liver B cells were cultured with spleen CD4+ T cells obtained from mice primed with heat-killed S. pneumoniae 7 d before, they induced IL-2 production and proliferation of the CD4+ T cells, along with Th1 cytokine production. However, they induced neither the CD4+ T cell production of IL-21, a suggested marker promoting B cell proliferation and differentiation, nor the expression of genes important for somatic hypermutation or isotype switching; such responses were particularly evident when splenic B cells merely capturing S. pneumoniae without processing them were cultured with spleen CD4+ T cells. These findings suggest that phagocytic liver B cells may be involved in acquired immune responses by presenting derivative peptides to CD4+ T cells without their own somatic hypermutation or isotype switching.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
卡列宁的微笑完成签到,获得积分10
刚刚
秦善斓完成签到,获得积分10
1秒前
1秒前
2秒前
2秒前
5秒前
阿紫发布了新的文献求助10
5秒前
6秒前
彭于彦祖应助丝叶狸藻采纳,获得50
6秒前
害羞外套发布了新的文献求助10
8秒前
ttjo完成签到 ,获得积分10
8秒前
光芒万丈发布了新的文献求助10
9秒前
9秒前
9秒前
banwo完成签到,获得积分20
10秒前
13秒前
卡卡罗特发布了新的文献求助30
13秒前
14秒前
科研通AI2S应助潇洒板凳采纳,获得10
15秒前
淑婷发布了新的文献求助10
15秒前
可爱的函函应助山河已秋采纳,获得10
15秒前
15秒前
Abner发布了新的文献求助10
18秒前
Bebetter完成签到,获得积分10
18秒前
21秒前
23秒前
23秒前
光芒万丈完成签到 ,获得积分10
24秒前
wanci应助阿紫采纳,获得10
25秒前
Lucas应助氨甲酰磷酸采纳,获得10
25秒前
C.Z.Young完成签到,获得积分10
27秒前
害羞外套完成签到,获得积分10
28秒前
JamesPei应助孔曼卉采纳,获得10
28秒前
VAN喵发布了新的文献求助10
28秒前
欣喜雅阳完成签到,获得积分10
28秒前
29秒前
29秒前
29秒前
30秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Mixed-anion Compounds 600
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Geochemistry, 2nd Edition 地球化学经典教科书第二版 401
Management of cardiopulmonary bypass 400
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3199162
求助须知:如何正确求助?哪些是违规求助? 2848029
关于积分的说明 8066187
捐赠科研通 2512827
什么是DOI,文献DOI怎么找? 1344946
科研通“疑难数据库(出版商)”最低求助积分说明 639938
邀请新用户注册赠送积分活动 609663