Mouse Liver B Cells Phagocytose <i>Streptococcus pneumoniae</i> and Initiate Immune Responses against Their Antigens

生物 免疫系统 体细胞突变 脾脏 肺炎链球菌 B-1电池 微生物学 吞噬作用 免疫球蛋白类转换 白细胞介素12 抗原 免疫学 B细胞 抗体
作者
Masahiro Nakashima,Manabu Kinoshita,Hiroyuki Nakashima,Azusa Kato,Kazuma Mori,Kazuki Koiwai,Nariyoshi Shinomiya,Shuhji Seki
出处
期刊:Journal of Immunology [The American Association of Immunologists]
卷期号:209 (1): 26-37
标识
DOI:10.4049/jimmunol.2100520
摘要

Recent studies have revealed that mammalian B cells ingest particulate Ags, such as bacteria, although little is known about the effect of this function on acquired immunity. We investigated the role of bacterium-phagocytosing B cells in acquired host immune responses. Cultured mouse liver B cells substantially phagocytosed serum-opsonized Streptococcus pneumoniae and produced IgM. On adoptive transfer of liver B cells that phagocytose S. pneumoniae labeled with pHrodo Red succinimidyl ester, recipient mice showed elevated plasma levels of IgG specific for bacterial Ags. In particular, the levels of IgG2a and IgG2b specific for pneumococcal surface protein A, as well as IgG3 for pneumococcal polysaccharide, were markedly increased compared with total IgG specific for each Ag. When phagocytic liver B cells were cultured with spleen CD4+ T cells obtained from mice primed with heat-killed S. pneumoniae 7 d before, they induced IL-2 production and proliferation of the CD4+ T cells, along with Th1 cytokine production. However, they induced neither the CD4+ T cell production of IL-21, a suggested marker promoting B cell proliferation and differentiation, nor the expression of genes important for somatic hypermutation or isotype switching; such responses were particularly evident when splenic B cells merely capturing S. pneumoniae without processing them were cultured with spleen CD4+ T cells. These findings suggest that phagocytic liver B cells may be involved in acquired immune responses by presenting derivative peptides to CD4+ T cells without their own somatic hypermutation or isotype switching.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
鱼鱼鱼发布了新的文献求助10
1秒前
感谢健壮冰淇淋转发科研通微信,获得积分50
1秒前
子车茗应助柯梦采纳,获得10
2秒前
Ekko完成签到,获得积分10
2秒前
星辰大海应助冰雪物语采纳,获得10
2秒前
2秒前
3秒前
wwy发布了新的文献求助10
3秒前
3秒前
4秒前
wyj发布了新的文献求助10
4秒前
5秒前
感谢薄荷喵转发科研通微信,获得积分50
5秒前
JamesPei应助双眸若星辰采纳,获得10
5秒前
memory完成签到,获得积分10
5秒前
小二郎应助小文采纳,获得10
6秒前
华仔应助研友_想想采纳,获得10
6秒前
6秒前
7秒前
李绿真发布了新的文献求助30
7秒前
K2L完成签到,获得积分10
8秒前
大力完成签到,获得积分10
9秒前
Maestro_S应助爱你不商量采纳,获得10
9秒前
9秒前
感谢i1转发科研通微信,获得积分50
10秒前
彭于晏应助眯眯眼的鞋垫采纳,获得10
10秒前
猛龙完成签到,获得积分10
10秒前
cc完成签到,获得积分10
10秒前
10秒前
Brian发布了新的文献求助10
11秒前
Owen应助科研通管家采纳,获得10
11秒前
小蘑菇应助科研通管家采纳,获得10
11秒前
深情安青应助科研通管家采纳,获得10
11秒前
科研通AI2S应助科研通管家采纳,获得10
11秒前
搜集达人应助科研通管家采纳,获得10
11秒前
Lucas应助科研通管家采纳,获得10
11秒前
Hello应助科研通管家采纳,获得10
11秒前
无花果应助科研通管家采纳,获得10
11秒前
12秒前
李健应助科研通管家采纳,获得10
12秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2024 Medicinal Chemistry Reviews 400
Dictionary of socialism 350
Mixed-anion Compounds 300
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3198246
求助须知:如何正确求助?哪些是违规求助? 2847229
关于积分的说明 8062837
捐赠科研通 2512120
什么是DOI,文献DOI怎么找? 1343954
科研通“疑难数据库(出版商)”最低求助积分说明 639678
邀请新用户注册赠送积分活动 609316