Mouse Liver B Cells Phagocytose <i>Streptococcus pneumoniae</i> and Initiate Immune Responses against Their Antigens

生物 免疫系统 体细胞突变 脾脏 肺炎链球菌 B-1电池 微生物学 吞噬作用 免疫球蛋白类转换 白细胞介素12 抗原 免疫学 B细胞 抗体
作者
Masahiro Nakashima,Manabu Kinoshita,Hiroyuki Nakashima,Azusa Kato,Kazuma Mori,Kazuki Koiwai,Nariyoshi Shinomiya,Shuhji Seki
出处
期刊:Journal of Immunology [The American Association of Immunologists]
卷期号:209 (1): 26-37
标识
DOI:10.4049/jimmunol.2100520
摘要

Recent studies have revealed that mammalian B cells ingest particulate Ags, such as bacteria, although little is known about the effect of this function on acquired immunity. We investigated the role of bacterium-phagocytosing B cells in acquired host immune responses. Cultured mouse liver B cells substantially phagocytosed serum-opsonized Streptococcus pneumoniae and produced IgM. On adoptive transfer of liver B cells that phagocytose S. pneumoniae labeled with pHrodo Red succinimidyl ester, recipient mice showed elevated plasma levels of IgG specific for bacterial Ags. In particular, the levels of IgG2a and IgG2b specific for pneumococcal surface protein A, as well as IgG3 for pneumococcal polysaccharide, were markedly increased compared with total IgG specific for each Ag. When phagocytic liver B cells were cultured with spleen CD4+ T cells obtained from mice primed with heat-killed S. pneumoniae 7 d before, they induced IL-2 production and proliferation of the CD4+ T cells, along with Th1 cytokine production. However, they induced neither the CD4+ T cell production of IL-21, a suggested marker promoting B cell proliferation and differentiation, nor the expression of genes important for somatic hypermutation or isotype switching; such responses were particularly evident when splenic B cells merely capturing S. pneumoniae without processing them were cultured with spleen CD4+ T cells. These findings suggest that phagocytic liver B cells may be involved in acquired immune responses by presenting derivative peptides to CD4+ T cells without their own somatic hypermutation or isotype switching.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
2秒前
科研强完成签到 ,获得积分10
2秒前
rr发布了新的文献求助10
2秒前
ycg完成签到,获得积分10
3秒前
JamesPei应助qing采纳,获得10
7秒前
Hello应助Ventus采纳,获得10
8秒前
Lilith完成签到 ,获得积分10
9秒前
aaa完成签到,获得积分10
9秒前
Gong完成签到,获得积分10
11秒前
丸子鱼完成签到 ,获得积分10
11秒前
MMMM完成签到,获得积分10
12秒前
小曹完成签到,获得积分10
15秒前
Sept完成签到,获得积分10
16秒前
空白完成签到,获得积分10
16秒前
ls完成签到 ,获得积分10
16秒前
蛙趣完成签到,获得积分10
17秒前
18秒前
沉静的灵安完成签到,获得积分20
19秒前
19秒前
21秒前
21秒前
外向一一发布了新的文献求助10
24秒前
wang完成签到,获得积分20
25秒前
lezbj99发布了新的文献求助10
26秒前
小七发布了新的文献求助10
27秒前
wanci应助不打游戏_采纳,获得10
28秒前
30秒前
HEIKU应助wang采纳,获得10
31秒前
科研通AI2S应助毕十三采纳,获得10
31秒前
科研通AI2S应助毕十三采纳,获得10
31秒前
内向的青荷完成签到,获得积分10
32秒前
哈哈哈完成签到 ,获得积分10
34秒前
小饭完成签到,获得积分10
35秒前
36秒前
36秒前
37秒前
解剖六楼那小哥完成签到 ,获得积分10
39秒前
Echo完成签到,获得积分0
39秒前
我服有点黑完成签到,获得积分10
40秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
Handbook on People's China (1957) 400
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3190354
求助须知:如何正确求助?哪些是违规求助? 2839625
关于积分的说明 8024732
捐赠科研通 2502549
什么是DOI,文献DOI怎么找? 1336609
科研通“疑难数据库(出版商)”最低求助积分说明 637841
邀请新用户注册赠送积分活动 606051