Mouse Liver B Cells Phagocytose <i>Streptococcus pneumoniae</i> and Initiate Immune Responses against Their Antigens

生物 免疫系统 体细胞突变 脾脏 肺炎链球菌 B-1电池 微生物学 吞噬作用 免疫球蛋白类转换 白细胞介素12 抗原 免疫学 B细胞 抗体
作者
Masahiro Nakashima,Manabu Kinoshita,Hiroyuki Nakashima,Azusa Kato,Kazuma Mori,Kazuki Koiwai,Nariyoshi Shinomiya,Shuhji Seki
出处
期刊:Journal of Immunology [The American Association of Immunologists]
卷期号:209 (1): 26-37
标识
DOI:10.4049/jimmunol.2100520
摘要

Recent studies have revealed that mammalian B cells ingest particulate Ags, such as bacteria, although little is known about the effect of this function on acquired immunity. We investigated the role of bacterium-phagocytosing B cells in acquired host immune responses. Cultured mouse liver B cells substantially phagocytosed serum-opsonized Streptococcus pneumoniae and produced IgM. On adoptive transfer of liver B cells that phagocytose S. pneumoniae labeled with pHrodo Red succinimidyl ester, recipient mice showed elevated plasma levels of IgG specific for bacterial Ags. In particular, the levels of IgG2a and IgG2b specific for pneumococcal surface protein A, as well as IgG3 for pneumococcal polysaccharide, were markedly increased compared with total IgG specific for each Ag. When phagocytic liver B cells were cultured with spleen CD4+ T cells obtained from mice primed with heat-killed S. pneumoniae 7 d before, they induced IL-2 production and proliferation of the CD4+ T cells, along with Th1 cytokine production. However, they induced neither the CD4+ T cell production of IL-21, a suggested marker promoting B cell proliferation and differentiation, nor the expression of genes important for somatic hypermutation or isotype switching; such responses were particularly evident when splenic B cells merely capturing S. pneumoniae without processing them were cultured with spleen CD4+ T cells. These findings suggest that phagocytic liver B cells may be involved in acquired immune responses by presenting derivative peptides to CD4+ T cells without their own somatic hypermutation or isotype switching.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
SciGPT应助regina采纳,获得10
1秒前
YL发布了新的文献求助10
1秒前
2秒前
善学以致用应助2023AKY采纳,获得10
2秒前
2秒前
调研昵称发布了新的文献求助10
3秒前
易寒发布了新的文献求助10
3秒前
情怀应助jdwkhevb采纳,获得10
3秒前
SciGPT应助科研通管家采纳,获得10
4秒前
4秒前
情怀应助科研通管家采纳,获得10
4秒前
4秒前
科研通AI2S应助科研通管家采纳,获得10
4秒前
4秒前
JJJJJJJ发布了新的文献求助10
5秒前
6秒前
7秒前
7秒前
星辰大海应助任梓宁采纳,获得10
8秒前
任性的冷梅完成签到,获得积分10
10秒前
星辰大海应助yhbk采纳,获得10
11秒前
12秒前
jdwkhevb发布了新的文献求助10
12秒前
领导范儿应助一月采纳,获得10
13秒前
FashionBoy应助张小鱼采纳,获得10
13秒前
李健应助YL采纳,获得10
13秒前
SciGPT应助bling采纳,获得10
13秒前
香蕉冰真完成签到 ,获得积分10
14秒前
Cheney完成签到 ,获得积分10
14秒前
邱帅发布了新的文献求助10
14秒前
huhu完成签到 ,获得积分10
15秒前
Orange应助好好好采纳,获得10
16秒前
16秒前
2023AKY发布了新的文献求助10
18秒前
m4a1akaug完成签到,获得积分10
19秒前
任梓宁发布了新的文献求助10
20秒前
21秒前
JJJJJJJ完成签到,获得积分20
21秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3182294
求助须知:如何正确求助?哪些是违规求助? 2832681
关于积分的说明 7990255
捐赠科研通 2494697
什么是DOI,文献DOI怎么找? 1330924
科研通“疑难数据库(出版商)”最低求助积分说明 636197
邀请新用户注册赠送积分活动 603124