Mouse Liver B Cells Phagocytose <i>Streptococcus pneumoniae</i> and Initiate Immune Responses against Their Antigens

生物 免疫系统 体细胞突变 脾脏 肺炎链球菌 B-1电池 微生物学 吞噬作用 免疫球蛋白类转换 白细胞介素12 抗原 免疫学 B细胞 抗体
作者
Masahiro Nakashima,Manabu Kinoshita,Hiroyuki Nakashima,Azusa Kato,Kazuma Mori,Kazuki Koiwai,Nariyoshi Shinomiya,Shuhji Seki
出处
期刊:Journal of Immunology [The American Association of Immunologists]
卷期号:209 (1): 26-37
标识
DOI:10.4049/jimmunol.2100520
摘要

Recent studies have revealed that mammalian B cells ingest particulate Ags, such as bacteria, although little is known about the effect of this function on acquired immunity. We investigated the role of bacterium-phagocytosing B cells in acquired host immune responses. Cultured mouse liver B cells substantially phagocytosed serum-opsonized Streptococcus pneumoniae and produced IgM. On adoptive transfer of liver B cells that phagocytose S. pneumoniae labeled with pHrodo Red succinimidyl ester, recipient mice showed elevated plasma levels of IgG specific for bacterial Ags. In particular, the levels of IgG2a and IgG2b specific for pneumococcal surface protein A, as well as IgG3 for pneumococcal polysaccharide, were markedly increased compared with total IgG specific for each Ag. When phagocytic liver B cells were cultured with spleen CD4+ T cells obtained from mice primed with heat-killed S. pneumoniae 7 d before, they induced IL-2 production and proliferation of the CD4+ T cells, along with Th1 cytokine production. However, they induced neither the CD4+ T cell production of IL-21, a suggested marker promoting B cell proliferation and differentiation, nor the expression of genes important for somatic hypermutation or isotype switching; such responses were particularly evident when splenic B cells merely capturing S. pneumoniae without processing them were cultured with spleen CD4+ T cells. These findings suggest that phagocytic liver B cells may be involved in acquired immune responses by presenting derivative peptides to CD4+ T cells without their own somatic hypermutation or isotype switching.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
loulex完成签到,获得积分10
4秒前
Dr_Shi完成签到,获得积分10
6秒前
Tina完成签到 ,获得积分10
7秒前
lalala发布了新的文献求助10
7秒前
adgfasdvz完成签到 ,获得积分10
10秒前
12秒前
ken131完成签到 ,获得积分10
12秒前
南风完成签到 ,获得积分10
14秒前
gzf完成签到 ,获得积分10
15秒前
slp完成签到 ,获得积分10
15秒前
16秒前
18秒前
丽莉发布了新的文献求助10
19秒前
竞鹤完成签到,获得积分10
21秒前
闫132完成签到,获得积分10
25秒前
C_Li完成签到,获得积分10
26秒前
Owen应助彩色的芷容采纳,获得10
27秒前
贾舒涵完成签到,获得积分10
27秒前
科目三应助丽莉采纳,获得10
28秒前
cistronic完成签到,获得积分10
32秒前
lalala发布了新的文献求助10
33秒前
qinqiny完成签到 ,获得积分10
38秒前
好名字完成签到 ,获得积分10
38秒前
smz完成签到 ,获得积分10
40秒前
在水一方应助彩色的芷容采纳,获得10
40秒前
榆木小鸟完成签到 ,获得积分10
41秒前
44秒前
香蕉觅云应助小九采纳,获得10
46秒前
49秒前
alho完成签到 ,获得积分10
50秒前
平淡松完成签到 ,获得积分10
51秒前
跳跃太清完成签到 ,获得积分10
53秒前
Dawn完成签到 ,获得积分10
53秒前
容容容完成签到 ,获得积分10
53秒前
54秒前
大白发布了新的文献求助10
57秒前
一禅完成签到 ,获得积分10
57秒前
59秒前
楚寅完成签到 ,获得积分10
1分钟前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Write Like a Chemist: A Guide and Resource (第二版) 600
Mixed-anion Compounds 600
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
Earth System Geophysics 500
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Geochemistry, 2nd Edition 地球化学经典教科书第二版 401
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3200810
求助须知:如何正确求助?哪些是违规求助? 2850571
关于积分的说明 8072470
捐赠科研通 2514308
什么是DOI,文献DOI怎么找? 1347060
科研通“疑难数据库(出版商)”最低求助积分说明 640332
邀请新用户注册赠送积分活动 610520