Online restricted access molecularly imprinted solid-phase extraction coupled with liquid chromatography-mass spectrometry for the selective determination of serum bile acids

色谱法 化学 分子印迹聚合物 检出限 分析物 牛血清白蛋白 质谱法 固相萃取 萃取(化学) 液相色谱-质谱法 牛磺酸 溶剂 氨基酸 生物化学 选择性 催化作用
作者
Alberto Thalison Silveira,Alyne Maria da Costa Barbosa,Henrique Dipe de Faria,Luiz Paulo de Aguiar Marciano,Eduardo Costa Figueiredo,Isarita Martins
出处
期刊:Analyst [Royal Society of Chemistry]
卷期号:147 (12): 2779-2792 被引量:8
标识
DOI:10.1039/d2an00446a
摘要

A rapid and sensitive online restricted access molecularly imprinted solid-phase extraction method coupled to a liquid chromatography-mass spectrometry (LC-MS) system for simultaneous determination of serum bile acids as well as their taurine and glycine conjugates was developed. Reversible liver damage of workers exposed to volatile organic solvents can be investigated based on the level of the analyzed molecules. A restricted access molecularly imprinted polymer coated with bovine serum albumin (RAMIP-BSA) was synthesized and used as the extraction phase. The column switching liquid chromatography system was able to exclude about 100% of the macromolecules and extract/separate nine bile acids from blood human serum samples, in a total time of 40 minutes. The developed method was validated based on the Food and Drug Administration (FDA) guidelines, being linear for all the analytes in their respective analytical ranges (coefficients of determination higher than 0.99), with limits of detection (LOD) and quantification (LOQ) ranging from 2.0 to 5.7 μg L-1 and from 10.0 to 25.0 μg L-1, respectively. Suitable results for precision (relative standard deviation ranged from 3.2 to 14.5% and 0.7 to 14.8%, respectively for intra and inter-assay) and accuracy (relative error ranged from -14.8 to 14.2%; -13.8 to 14.3%) were obtained. The validated analytical method was applied to determine bile acids in serum samples of five workers occupationally exposed to volatile organic solvent, demonstrating its applicability in the assessment of these toxicants.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
淡定的依瑶完成签到,获得积分10
1秒前
江璃发布了新的文献求助10
3秒前
4秒前
5秒前
美丽的安珊完成签到,获得积分10
6秒前
6秒前
8秒前
Gilana完成签到,获得积分10
8秒前
xyh发布了新的文献求助10
8秒前
江璃完成签到,获得积分10
9秒前
TT发布了新的文献求助10
9秒前
美梦成真完成签到,获得积分10
10秒前
Gakay完成签到,获得积分10
10秒前
量子星尘发布了新的文献求助10
11秒前
szj完成签到,获得积分0
12秒前
旦皋完成签到,获得积分10
12秒前
赘婿应助花壳在逃野猪采纳,获得10
13秒前
卷卷完成签到,获得积分10
15秒前
JSY完成签到 ,获得积分20
15秒前
xyh完成签到,获得积分10
16秒前
小曾应助Florencia采纳,获得10
17秒前
神外王001完成签到 ,获得积分10
17秒前
22秒前
你是谁完成签到,获得积分10
23秒前
majf完成签到,获得积分10
24秒前
linhanwenzhou完成签到,获得积分10
24秒前
JSY关注了科研通微信公众号
24秒前
853225598完成签到,获得积分10
24秒前
798完成签到,获得积分10
25秒前
善学以致用应助董怼怼采纳,获得10
25秒前
妍儿完成签到,获得积分20
26秒前
隐形曼青应助高大的水壶采纳,获得10
26秒前
马哥二弟无敌完成签到 ,获得积分10
27秒前
28秒前
Florencia完成签到,获得积分10
28秒前
务实颜完成签到 ,获得积分10
28秒前
28秒前
amberzyc应助小远采纳,获得10
29秒前
29秒前
30秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029