MAPK/ERK通路
车站3
STAT蛋白
细胞生物学
激酶
免疫印迹
电泳迁移率测定
信号转导
化学
分子生物学
信使核糖核酸
转录因子
免疫荧光
生物
基因
免疫学
抗体
生物化学
作者
Woo In Ryu,Hana Lee,Hyun Cheol Bae,Jiehyun Jeon,Hwa Jung Ryu,Jaehyung Kim,Ji Hyun Kim,Ji‐Won Son,Jae Young Kim,Yasutomo Imai,Kiyofumi Yamanishi,Sang Hoon Jeong,Sang Wook Son
标识
DOI:10.1016/j.jdermsci.2018.02.017
摘要
Background Tight junctions (TJs) have important roles in skin barrier function. The TJ protein claudin-1 (CLDN1) is decreased in atopic dermatitis (AD). However, little is known about the mechanism of CLDN1 down-expression. Objective To elucidate the effect of IL-33 on CLDN1 expression in keratinocytes. Methods Normal human epidermal keratinocytes (NHEKs) and human skin equivalent models (HSEMs) were cultured in vitro in the presence of IL-33. Production of CLDN1, signal transducer and activator of transcription 3 (STAT3) and Mitogen-activated protein kinases (MAPK) expression were measured by real-time PCR, western blot and immunofluorescence assay. MAPK inhibitors and small interfering RNA were used to confirm the signal pathway of STAT3 and CLDN1. Barrier function was measured by transepithelial electric resistance (TEER) and FITC-dextran flux assays. Electrophoretic Mobility Shift Assay was used to detect STAT3 transcriptional activity. Results Levels of CLDN1 expression were reduced in the epidermis of AD-model mice overexpressing IL-33. IL-33 down-regulated the expression of CLDN1 mRNA and protein in NHEKs and HSEMs. IL-33 attenuated transepithelial electric resistance and induced FITC-dextran flux in NHEKs. The IL-33 suppressed CLDN1 expression was regulated by an extracellular signal-regulated kinase (ERK) and signal transducer and activator of transcription 3 (STAT3). STAT3 suppressed CLDN1 expression by direct binding to the promoters. Conclusion IL-33 may down-regulate CLDN1 expression through the ERK/STAT3 pathway in keratinocytes.
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