生物
遗传学
基因
大块分离分析
候选基因
枯萎病
植物抗病性
编码区
分子标记
基因定位
植物
染色体
作者
Ruikang K. Wang,Yan Lin,Chunlong Zhang,Haibin Wu,Qiang Jin,Jiguang Guo,Haishun Cao,Tan Dai,Tingquan Wu
出处
期刊:Plant Biology
[Wiley]
日期:2024-04-12
卷期号:26 (4): 583-591
被引量:1
摘要
ABSTRACT Cucumber blight is a destructive disease. The best way to control this disease is resistance breeding. This study focuses on disease resistance gene mapping and molecular marker development. We used the resistant cucumber, JSH, and susceptible cucumber, B80, as parents to construct F2 populations. Bulked segregant analysis (BSA) combined with specific length amplified fragment sequencing (SLAF‐seq) were used, from which we developed cleaved amplified polymorphic sequence (CAPs) markers to map the resistance gene. Resistance in F2 individuals showed a segregation ratio of resistance:susceptibility close to 3:1. The gene in JSH resistant cucumber was mapped to an interval of 9.25 kb, and sequencing results for the three genes in the mapped region revealed three mutations at base sites 225, 302, and 591 in the coding region of Csa5G139130 between JSH and B80, but no mutations in coding regions of Csa5G139140 and Csa5G139150 . The mutations caused changes in amino acids 75 and 101 of the protein encoded by Csa5G139130 , suggesting that Csa5G139130 is the most likely resistance candidate gene. We developed a molecular marker, CAPs‐4, as a closely linked marker for the cucumber blight resistance gene. This is the first report on mapping of a cucumber blight resistance gene and will provideg a useful marker for molecular breeding of cucumber resistance to Phytophthora blight.
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