清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

3-Aminophenylboronic Acid Conjugation on Responsive Polymer and Gold Nanoparticles for Qualitative Bacterial Detection

胶体金 吸光度 Zeta电位 表面等离子共振 硼酸 聚合物 纳米颗粒 化学 丙烯酸 分光光度法 聚合 粒径 纳米技术 材料科学 核化学 色谱法 组合化学 共聚物 有机化学 物理化学
作者
Erindyah Retno Wikantyasning,Muhammad Da’i,Zakky Cholisoh,Ummi Kalsum
出处
期刊:Journal of Pharmacy and Bioallied Sciences [Medknow Publications]
卷期号:15 (2): 81-87 被引量:1
标识
DOI:10.4103/jpbs.jpbs_646_22
摘要

Because of their sensitive and selective responses to a wide variety of analytes, colorimetric sensors have gained widespread acceptance in recent years. Gold nanoparticles (AuNPs) are widely employed in visual sensor strategies due to their high stability and ease of use. Combining AuNPs with a responsive polymer can result in distinct surface plasmon resonance (SPR) changes that can be utilized as colorimetric biosensors.The purpose of this research is to develop a colorimetric-based sensor through the utilization of the optical properties of gold nanoparticles (AuNPs) crosslinked with pH-responsive polymers poly (acrylic acid) (PAA) conjugated to 3-aminophenyl boronic acid (APBA).The polymer (PAA) was synthesized via RAFT polymerization. The inversed Turkevic method was used to produce AuNPs, which were subsequently used in a self-assembly process using poly (acrylic acid)-aminophenyl boronic acid (PAA-APBA) to create the self-assembled AuNPs-APBA-PAA. The particle size, zeta potential, and reversibility of the polymer-modified gold nanoparticles were determined using a transmission electron microscope (TEM), a particle size analyzer (PSA), and an Ultraviolet-Visible spectrophotometer (UV-Vis spectrophotometer). Visual, UV-Vis spectrophotometer and TEM observations confirmed the system's ability to identify bacteria. Statistical analysis was performed using a one-way analysis of variance using Excel software.Using UV-Vis spectrophotometry, the particle size of AuNPs was determined to be 25.7 nm, and the maximum absorbance occurred at 530 nm. AuNPs PAA APBA colloid exhibited an absorbance maximum of 532 nm, a zeta potential of -41.53, and a pH transition point between 4 and 5. At E. coli concentrations of 4.5 x 107 CFU/mL, the color of the system sensors changed from red to blue after 15 hours of incubation, whereas at S. aureus concentrations of 1.2 x 109 CFU/mL, the color changed to purple immediately after mixing. The TEM confirmed that the detection mechanism is based on the boronate-polyol bonding of saccharides on the outer membranes of Escherichia coli and Staphylococcus aureus.The use of APBA in conjunction with pH-responsive PAA polymers containing AuNPs to detect E. coli and S. aureus bacteria induces a maximum wavelength transition, followed by a color change from red to blue. By the process of de-swelling of the responsive polymer, which induces the aggregation of the AuNPs, the established sensor system is able to alter the color. The conjugated polymer and gold nanoparticle-based sensor system demonstrated a promising method for bacterial detection.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Nan发布了新的文献求助10
1秒前
拓跋雨梅完成签到 ,获得积分0
15秒前
赘婿应助咕咕咕咕采纳,获得10
1分钟前
月军完成签到,获得积分10
1分钟前
2分钟前
Qing完成签到 ,获得积分10
2分钟前
3分钟前
咕咕咕咕发布了新的文献求助10
3分钟前
3分钟前
在水一方完成签到 ,获得积分0
6分钟前
肆肆完成签到,获得积分10
7分钟前
脆饼同学完成签到 ,获得积分10
7分钟前
8分钟前
8分钟前
qinxy发布了新的文献求助10
9分钟前
宇文非笑完成签到 ,获得积分10
9分钟前
执着夏山完成签到,获得积分10
9分钟前
qinxy完成签到 ,获得积分10
11分钟前
迷茫的一代完成签到,获得积分10
11分钟前
12分钟前
金钰贝儿完成签到,获得积分10
12分钟前
12分钟前
我是雷锋发布了新的文献求助10
12分钟前
12分钟前
Emperor完成签到 ,获得积分0
13分钟前
我是雷锋完成签到,获得积分10
13分钟前
小烦同学完成签到,获得积分10
13分钟前
浪麻麻完成签到 ,获得积分10
13分钟前
古炮完成签到 ,获得积分10
13分钟前
ww完成签到,获得积分10
14分钟前
Demi_Ming完成签到,获得积分10
14分钟前
77完成签到 ,获得积分10
14分钟前
kk完成签到,获得积分10
14分钟前
睡到自然醒完成签到 ,获得积分10
15分钟前
16分钟前
xiaoqingnian完成签到,获得积分10
16分钟前
隔壁的镇长完成签到,获得积分10
16分钟前
明理问柳完成签到,获得积分10
17分钟前
不如一默完成签到,获得积分10
17分钟前
19分钟前
高分求助中
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
Дружба 友好报 (1957-1958) 1000
The Data Economy: Tools and Applications 1000
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufe 700
A Dissection Guide & Atlas to the Rabbit 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3099799
求助须知:如何正确求助?哪些是违规求助? 2751268
关于积分的说明 7612112
捐赠科研通 2403028
什么是DOI,文献DOI怎么找? 1275146
科研通“疑难数据库(出版商)”最低求助积分说明 616263
版权声明 599053