耐旱性
脱落酸
RNA干扰
生物
蛋白磷酸酶2
限制
磷酸酶
适应性
基因
基因沉默
适应(眼睛)
细胞生物学
下调和上调
植物
核糖核酸
生物化学
磷酸化
生态学
工程类
机械工程
神经科学
作者
Jianhui Ma,Yuke Geng,Hong Liu,Mengqi Zhang,Shujuan Liu,Chenyang Hao,Jian Hou,Youfu Zhang,Daijing Zhang,Weijun Zhang,Xueyong Zhang,Tian Li
摘要
Drought is a major environmental stress limiting global wheat (Triticum aestivum) production. Exploring drought tolerance genes is important for improving drought adaptation in this crop. Here, we cloned and characterized TaTIP41, a novel drought tolerance gene in wheat. TaTIP41 is a putative conserved component of target of rapamycin (TOR) signaling, and the TaTIP41 homoeologs were expressed in response to drought stress and abscisic acid (ABA). The overexpression of TaTIP41 enhanced drought tolerance and the ABA response, including ABA-induced stomatal closure, while its downregulation using RNA interference (RNAi) had the opposite effect. Furthermore, TaTIP41 physically interacted with TaTAP46, another conserved component of TOR signaling. Like TaTIP41, TaTAP46 positively regulated drought tolerance. Furthermore, TaTIP41 and TaTAP46 interacted with type-2A protein phosphatase (PP2A) catalytic subunits, such as TaPP2A-2, and inhibited their enzymatic activities. Silencing TaPP2A-2 improved drought tolerance in wheat. Together, our findings provide new insights into the roles of TaTIP41 and TaTAP46 in the drought tolerance and ABA response in wheat, and their potential application in improving wheat environmental adaptability.
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