介电谱
缓冲器(光纤)
电极
万古霉素
信号(编程语言)
电阻抗
材料科学
孵化
缓冲溶液
光电子学
磷酸盐缓冲盐水
电化学
线性范围
分析化学(期刊)
生物医学工程
化学
电气工程
色谱法
检出限
计算机科学
电信
医学
工程类
物理化学
生物化学
程序设计语言
细菌
遗传学
金黄色葡萄球菌
生物
作者
Buddhadev Purohit,Winnie Edith Svendsen
摘要
Disposable screen-printed gold electrodes (SPAuEs) are used for the development of numerous immunosensors owing to its ease of modification with biorecognition molecules. A major limitation in development of SPAuEs based biosensor is the single-use of the electrodes leading to higher costs for sensor development, whereas multiple measurements can affect the sensor stability and reproducibility. Especially, label-free electrochemical impedance spectroscopy (EIS) based SPAuEs sensors are prone to signal drift resulting in false-positive or false-negative data questioning the reliability of the assay. We found that repeated EIS measurements on SPAuEs in ferri- and ferrocyanide solution yielded a significant increase in resistance to charge transfer (Rct) value (positive signal drift) even in a control solution (without the target analyte). The positive signal drift follows the typical pattern of a concentration-dependent calibration curve, rendering repeated measurement error-prone for sensing purposes. In this study, it is shown that the incubation of the SPAuEs in nitrogen purged deaerated phosphate buffer minimized this signal drift in repeated/multiple EIS measurements. This method was then used to develop a peptide-based EIS biosensor for the detection of Vancomycin, a last-line antibiotic used for the treatment of severe multidrug-resistant bacterial infection. This protocol can be followed for the biosensing of other clinically important biomarkers.
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