化学
化学物理
分子
化学成分
作文(语言)
核磁共振波谱
分子动力学
组分(热力学)
计算化学
有机化学
物理
热力学
语言学
哲学
作者
Christian F. Pantoja,Alain Ibáñez de Opakua,Maria‐Sol Cima‐Omori,Markus Zweckstetter
标识
DOI:10.1002/anie.202218078
摘要
Liquid-Liquid phase separation has emerged as fundamental process underlying the formation of biomolecular condensates. Insights into the composition and structure of biomolecular condensates is, however, complicated by their molecular complexity and dynamics. Here, we introduce an improved spatially-resolved NMR experiment that enables quantitative analysis of the physico-chemical composition of multi-component biomolecular condensates in equilibrium and label-free. Application of spatially-resolved NMR to condensates formed by the Alzheimer's disease-associated protein Tau demonstrates decreased water content, exclusion of the molecular crowding agent dextran, presence of a specific chemical environment of the small molecule DSS, and ≈150-fold increased concentration of Tau inside the condensate. The results suggest that spatially-resolved NMR can have a major impact in understanding the composition and physical chemistry of biomolecular condensates.
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