HIV-1 RNA and DNA Genotyping Drug Resistance Detection in Patients with Low-Level Viremia in Liangshan, China

基因分型 病毒血症 抗药性 病毒学 逆转录酶 生物 病毒载量 艾滋病毒耐药性 核糖核酸 慢病毒 核苷逆转录酶抑制剂 人类免疫缺陷病毒(HIV) 基因型 基因 病毒性疾病 抗逆转录病毒疗法 遗传学
作者
Bianchuan Cao,Mei Liu,Tao Jiang,Qinghua Yu,Tianru Yuan,Ping Ding,Xian Zhou,Fuli Huang,Yongmao Huang,Jianning Jiang
出处
期刊:AIDS Research and Human Retroviruses [Mary Ann Liebert, Inc.]
卷期号:39 (8): 429-435 被引量:4
标识
DOI:10.1089/aid.2022.0140
摘要

In this study, we characterized HIV-1 RNA and HIV-1 DNA genotyping drug resistance detection in patients with low-level viremia (LLV) in Liangshan, China. Whole blood samples were collected from HIV/AIDS patients who had received antiretroviral therapy (ART) for ≥6 months and whose HIV-1 RNA loads were 50-1,000 copies/mL for two consecutive times at least 1-month apart. The patients were enrolled from a county in Liangshan Yi Autonomous Prefecture, Sichuan Province, between May 2021 and May 2022. Plasma and blood cells were separated. Plasma samples were tested for HIV-1 RNA genotyping drug resistance, while blood cell samples were tested for HIV-1 DNA genotyping drug resistance. Then, HIV-1 RNA and HIV-1 DNA genotyping drug resistance outcomes were compared. Among the 32 participants, 16 were males, while 16 were females, with the median age of 34.5 years. The main HIV-1 infection route was heterosexual transmission. The median ART duration was 3.9 years. Two types of nucleoside reverse transcriptase inhibitors (NRTIs) + one non-nucleoside reverse transcriptase inhibitor (NNRTI) were the main antiviral therapeutic options. Pol region genes for 28 HIV-1 DNA samples and 10 HIV-1 RNA samples were successfully amplified. The success rate of pol region gene amplification for HIV-1 DNA was significantly higher than that of HIV-1 RNA (χ2 = 20.988, p < .05). In HIV-1 RNA and HIV-1 DNA samples, M184 (4/8) and K103 (3/8) were the most frequent drug resistance mutation sites. Among the NNRTIs, the rates of drug resistance were highest to efavirenz (EFV) (6/8) and nevirapine (NVP) (6/8), while among the NRTIs, the rates of drug resistance were highest to abacavir (ABC) (4/8), emtricitabine (FTC) (4/8), and lamivudine (3TC) (4/8). In conclusion, detection of HIV-1 RNA genotyping drug resistance combined with HIV-1 DNA genotyping drug resistance can improve the success rate of drug resistance detection in patients with LLV.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
秋枫忆完成签到,获得积分10
2秒前
宋立发布了新的文献求助10
2秒前
孤独的AD钙完成签到,获得积分10
2秒前
3秒前
fang应助miao采纳,获得10
4秒前
星辰与月完成签到,获得积分10
4秒前
Pt-SACs发布了新的文献求助10
4秒前
安静无招完成签到 ,获得积分10
8秒前
lqphysics完成签到,获得积分10
9秒前
Jerry完成签到 ,获得积分10
9秒前
枕星发布了新的文献求助10
9秒前
全职法师刘海柱完成签到,获得积分10
11秒前
Umar完成签到,获得积分10
11秒前
accepted完成签到,获得积分10
12秒前
13秒前
Pt-SACs完成签到,获得积分10
14秒前
wgglegg完成签到 ,获得积分10
14秒前
饱满跳跳糖完成签到,获得积分10
14秒前
zhaoxiaonuan完成签到,获得积分10
14秒前
15秒前
赘婿应助2023204306324采纳,获得10
15秒前
英勇笑萍完成签到,获得积分10
16秒前
yar完成签到 ,获得积分10
17秒前
18秒前
伦语发布了新的文献求助10
18秒前
沉静怜蕾完成签到,获得积分10
18秒前
18秒前
欢呼白晴完成签到 ,获得积分10
19秒前
凉白开发布了新的文献求助10
19秒前
金扇扇完成签到 ,获得积分10
19秒前
Odyssey_Cheung完成签到,获得积分10
20秒前
JG完成签到,获得积分10
20秒前
明亮紫易完成签到,获得积分10
21秒前
张绪帆完成签到,获得积分10
21秒前
22秒前
龙眼完成签到,获得积分10
22秒前
clientprogram完成签到,获得积分0
22秒前
wendy发布了新的文献求助10
22秒前
量子星尘发布了新的文献求助10
23秒前
23秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029