磷光
铱
线粒体
化学
生物相容性
光化学
红外线的
亚硫酸氢盐
检出限
组合化学
生物物理学
生物化学
催化作用
荧光
有机化学
生物
光学
物理
基因表达
基因
DNA甲基化
色谱法
作者
Haotian Xin,Yuzhen Huang,Yuan Han,Luyao Tang,Guiyi Yang,Yan Zhang,Songfang Zhao,Kang‐Nan Wang,Yibing Li,Duxia Cao
标识
DOI:10.1016/j.saa.2023.122876
摘要
The derivatives of sulfur dioxide (HSO3-) formed in the biological environment play a vital role in the circulation system. Excessive SO2 derivatives will cause serious damage to the living system. Herein, a two-photon phosphorescent probe based on Ir(III) complex (named as Ir-CN) was designed and synthesized. Ir-CN is extremely selective and sensitive to SO2 derivatives with significant phosphorescent enhancement and increased phosphorescent lifetime. The detection limit of Ir-CN for SO2 derivatives reaches 0.17 μM. More importantly, Ir-CN preferentially accumulates in mitochondria, so bisulfite derivatives can be detected at subcellular level, which enriching the application of metal complex probe in biological detection. In addition, both single-photon and two-photon images can clearly show that Ir-CN is targeted to mitochondria. Benefits from its good biocompatibility, Ir-CN may be used as a reliable tool to detect SO2 derivatives in mitochondrion of living cells.
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