酿酒酵母
发酵
生产(经济)
过程(计算)
代谢工程
生物技术
生物化学
生化工程
化学
生物
酵母
计算机科学
酶
工程类
经济
宏观经济学
操作系统
作者
Pan Feng,Bowen Sun,Haoran Bi,Yufei Bao,Meng Wang,Huili Zhang,Yunming Fang
标识
DOI:10.1021/acssynbio.4c00728
摘要
α-Bisabolene's distinctive aroma is highly prized in fragrances and cosmetics, while its antioxidant properties hold significant pharmaceutical potential. However, the production of α-bisabolene in Saccharomyces cerevisiae remains an outstanding challenge due to cell growth limitations and insufficient supply of the α-bisabolene precursor farnesyl pyrophosphate. In this work, a new S. cerevisiae platform strain capable of producing high levels of α-bisabolene was presented. Carbon flux in the α-bisabolene synthesis pathway was maximized by iterative enhancement of the mevalonate metabolic pathway. The effects of MVA pathway intermediates on cell growth were addressed through a two-stage fermentation controlled based on a temperature-sensitive regulation strategy. The fermentation medium was optimized based on metabolomics and response surface model analysis. Under the optimal fermentation process, the titer of α-bisabolene reached 18.6 g/L during fed-batch fermentation, representing the highest titer reported to date. These strategies open up new avenues for industrial-scale terpene biosynthesis.
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