炎症
骨关节炎
NF-κB
化学
软骨
细胞外基质
促炎细胞因子
αBκ
细胞生物学
NFKB1型
肿瘤坏死因子α
癌症研究
药理学
免疫学
医学
转录因子
病理
生物化学
生物
解剖
基因
替代医学
作者
Peng Zhang,Yesheng Jin,Wei Xia,Xiaotong Wang,Zhiqiang Zhou
标识
DOI:10.1016/j.jot.2023.03.002
摘要
Osteoarthritis (OA), widely seen in the elderly, is featured by cartilage degradation, subchondral bone remolding, and synovium inflammation. Currently, there is no cure for OA development. Phillygenin (PHI), an active ingredient from the Forsythiae Fructus, possesses many biological properties, such as anti-inflammation and anti-oxidative stress in several diseases. However, the potential effects and underlying mechanisms of PHI on OA remain unclear. Western blotting, RT-PCR, ELISA and tissue staining were employed to explore the mechanisms by which PHI exerted a protective effect on IL-1β-induced production of pro-inflammation cytokines and extracellular matrix (ECM) degradation in primary murine chondrocytes and destabilization of the medial meniscus (DMM) mouse models. In this study, we found that PHI inhibited the production of pro-inflammation cytokines and ECM degradation induced by IL-1β in primary murine chondrocytes. Mechanically, PHI inhibited the NF-κB pathway via activating nuclear factor (erythrluteolind-derived 2)-like 2 (Nrf2). In vivo experiments also confirmed the chondroprotection of PHI in DMM mouse models. PHI alleviated IL-1β-induced inflammation cytokines and ECM degradation via activating Nrf2 and inhibiting NF-κB pathway. This study provides a biological rationale for the use of PHI as a potential candidate for OA treatment.
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