体内
谷胱甘肽
检出限
荧光
发光
半胱氨酸
斯托克斯位移
杂蒽
光化学
化学
材料科学
光电子学
光学
生物
生物化学
物理
酶
色谱法
生物技术
作者
Yongquan Wu,Xinfeng Guo,Xiao Ma,Yongtian Zhu,Yi Liu,Hong Zeng
标识
DOI:10.1016/j.snb.2023.133705
摘要
Biothiols including cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play important roles in the antioxidant activity of cells, and a variety of diseases could be linked to their abnormal levels. Frequency up-conversion luminescence (FUCL) probes have low background fluorescence and cause low tissue damage; thus it is less harmful than Stokes shift luminescence probes, the traditional fluorescence probes. However, the utilization of FUCL probes for detection of biothiols has rarely been reported. In this work, we designed and synthesized a near-infrared (NIR) xanthene derivative IFU-1 and used as a frequency up-conversion luminescence probe to detect biothiols. The probe IFU-1 had high selectivity for biothiols and exhibited enhanced FUCL signals in response to them. In addition, it has extremely low limit of detection with regards to different biothiols: 4.8 nM for Cys; 6.2 nM for Hcy, and 9.5 nM for GSH, which are about 10 times lower than the detection limit of traditional excitation methods (63 nM for Cys, 31 nM for Hcy, and 76 nM for GSH). This work presents a novel approach for constructing FUCL biosensors.
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