苯丙素
生物合成
小桶
发芽
苯丙氨酸
生物
生物化学
鬼针草
代谢途径
转录组
酶
植物
化学
基因
基因表达
氨基酸
作者
Yao Tong,Shan-Chi Yi,Shu-Yu Liu,Lin Xu,Zhuo-Xun Qiu,Dongqiang Zeng,Wenwei Tang
标识
DOI:10.1016/j.ecoenv.2022.113943
摘要
Bruceine D is a natural quassinoid, which was successfully isolated in our research group from the residue of Brucea javanica (L.) seeds. Our previous research showed that Bruceine D prevented Bidens pilosa L. seed germination by suppressing the activity of key enzymes and the expression levels of key genes involved in the phenylpropanoid biosynthesis pathway. In this study, integrated analyses of non-targeted metabolomic and transcriptomic were performed. A total of 356 different accumulated metabolites (DAMs) were identified, and KEGG pathway analyses revealed that most of these DAMs were involved in phenylpropanoid biosynthesis. The decreased expression of ADTs and content of L-phenylalanine implicates that Bruceine D may suppress the downstream phenylpropanoid biosynthesis pathway by disrupting primary metabolism, that is, the phenylalanine biosynthesis pathway, thus inhibiting the final products, resulting in the interruption of B. pilosa seed germination. These results suggest that Bruceine D may inhibit the B. pilosa seed germination by suppressing phenylpropanoid biosynthesis through acting on ADTs.
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