流出
铜绿假单胞菌
微生物学
生物
环丙沙星
抗生素
抗生素耐药性
琼脂稀释
美罗培南
多重耐药
抗药性
细菌
最小抑制浓度
生物化学
遗传学
作者
Abed Zahedi Bialvaei,Mohammad Rahbar,Ramin Hamidi-Farahani,Ali Asgari,Aylin Esmailkhani,Yasaman Mardani dashti,Saeed Soleiman-Meigooni
标识
DOI:10.1016/j.micpath.2021.104789
摘要
Resistance-Nodulation-Division (RND) efflux pumps are responsible for multidrug resistance in Pseudomonas aeruginosa. The present study aimed to evaluate the overexpression of RND efflux pumps and its role in the antibiotic resistance of P. aeruginosa clinical isolates. A number of 122 isolates were obtained from three military hospitals in Tehran, Iran. In order to determine the antibiotic resistance, the isolates were identified and assessed by the disk diffusion and agar dilution methods. This study investigated the gene expression of four multi-drug efflux pump systems (MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY (-OprA)) and its correlation with antibiotic resistance. The isolates indicated that the highest resistance rate was against ticarcillin (80%), followed by ciprofloxacin (74%) and meropenem (71%). Most of them expressed mexB (69%), mexC (28.7%), mexE (43.4%), and mexY (74.6%), suggesting that mexB and mexY were highly expressed in the studied strains. The overexpression of mexB and mexY was significantly more prevalent in the ICU wards (p = 0.033). Furthermore, there was a significant correlation between the expression of RND-type efflux pumps and the resistance to most anti-pseudomonal antibiotics.
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