亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Effect of San'ao decoction with scorpio and bombyx batryticatus on CVA mice model via airway inflammation and regulation of TRPA1/TRPV1/TRPV5 channels

医学 TRPV1型 炎症 药理学 汤剂 气道高反应性 动物模型 化学 传统医学 内科学 受体 瞬时受体电位通道
作者
Pengli Wang,Erxin Shang,Xinsheng Fan
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:264: 113342-113342 被引量:23
标识
DOI:10.1016/j.jep.2020.113342
摘要

Cough variant asthma (CVA) is characterized with its long-lasting cough symptom on clinic. The mechanism of CVA is related to chronic persistent airway inflammation, airway hyperresponsiveness, etc. The traditional Chinese prescription has achieved good curative effect on CVA treatment through reducing cough counts, decreasing airway hyperresponsiveness and alleviating airway inflammation. The mechanism is associated with reducing IL4, IL-13, NGF and CGRP levels, as well as down-regulating TRPA1/TRPV1/TRPV5 channels in both lung and brain tissues. The Chinese prescription, San'ao decoction with scorpio and bombyx batryticatus (SSB), is well known in treating cough in asthmatic patients. In this study, the anti-tussive and anti-asthmatic role of SSB, as well as its mechanism on CVA mice model were explored and evaluated via alleviating airway inflammation and regulation of TRP channels. The major chemical components in SSB were detected and analyzed by UPLC-QTOF-MS under an optimized chromatographic and MS condition. 60 BALB/c mice were randomly divided into six groups: normal group, model group, dexamethasone group (0.1178 mg/kg/d), SSB high dose group (9.74 g/kg/d), SSB middle dose group (4.87 g/kg/d) and SSB low dose group (2.435 g/kg/d). The cough variant asthma mice model was established by ovalbumin sensitization and challenge. The protective role of SSB on CVA mice model was studied through inducing cough counts by capsaicin, assessing inflammatory cells in peripheral blood and bronchoalveolar lavage fluid (BALF), measuring airway responsiveness, detecting histopathological changes in lung tissues, analyzing cytokines and neuropeptides levels in BALF, as well as examining the mRNA and protein expressions of TRPA1, TRPV1 and TRPV5 in both lung and brain tissues. 17 signal peaks of the chemical components in SSB were identified by using UPLC-QTOF-MS. SSB (especially the high dose and middle dose), showed significantly effects on mice model by reducing mice cough counts (P < 0.01), decreasing eosinophil (EOS) counts in blood (P < 0.01) and inflammatory cell numbers in BALF (P < 0.01), decreasing airway hyperresponsiveness (P < 0.05), reducing the levels of IL-4 (P < 0.05), IL-13 (P < 0.01), NGF (P < 0.01) and CGRP (P < 0.01) in BALF, as well as down regulating the mRNA and protein expressions of TRPA1, TRPV1 and TRPV5 in both lung and brain tissues (P < 0.01). SSB showed anti-tussive and anti-asthmatic effects on cough variant asthma mice model by reducing cough counts, improving lung function, alleviating lung injury and airway inflammation. The mechanism of SSB might be associated with the regulation of cytokines and neuropeptides in BALF, as well as the regulation of TRPA1, TRPV1, TRPV5 channels in both lung and brain tissues.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
slayers完成签到 ,获得积分20
7秒前
Billy完成签到,获得积分0
16秒前
19秒前
20秒前
22秒前
SYLH应助Billy采纳,获得10
26秒前
量子星尘发布了新的文献求助10
36秒前
lascqy完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
优秀黑夜发布了新的文献求助10
1分钟前
Ava应助优秀黑夜采纳,获得10
1分钟前
冰留完成签到 ,获得积分10
1分钟前
1分钟前
量子星尘发布了新的文献求助10
2分钟前
852应助feiying采纳,获得10
2分钟前
无花果应助breeze采纳,获得10
2分钟前
隐形曼青应助Master采纳,获得10
3分钟前
3分钟前
Master发布了新的文献求助10
3分钟前
3分钟前
量子星尘发布了新的文献求助10
3分钟前
领导范儿应助科研通管家采纳,获得10
3分钟前
科研通AI2S应助科研通管家采纳,获得10
3分钟前
al完成签到 ,获得积分10
3分钟前
3分钟前
早早发布了新的文献求助10
3分钟前
科研通AI5应助Djnsbj采纳,获得10
4分钟前
4分钟前
量子星尘发布了新的文献求助10
5分钟前
5分钟前
顺心蜜粉发布了新的文献求助200
5分钟前
5分钟前
Djnsbj发布了新的文献求助10
5分钟前
5分钟前
breeze发布了新的文献求助10
5分钟前
我是老大应助科研通管家采纳,获得10
5分钟前
Daisy完成签到,获得积分10
5分钟前
6分钟前
量子星尘发布了新的文献求助150
6分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976665
求助须知:如何正确求助?哪些是违规求助? 3520756
关于积分的说明 11204743
捐赠科研通 3257502
什么是DOI,文献DOI怎么找? 1798733
邀请新用户注册赠送积分活动 877897
科研通“疑难数据库(出版商)”最低求助积分说明 806629