Detection of Myxobolus rotundus (Myxozoa: Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 54:171-173 (2003) - doi:10.3354/dao054171 Detection of Myxobolus rotundus (Myxozoa: Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody Y. S. Lu, P. Nie*, B. J. Sun State Key Laboratory of Freshwater Ecology and Biotechnology and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, PR China *Corresponding author. Email: pinnie@ihb.ac.cn ABSTRACT: Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish. KEY WORDS: Myxosporean · Myxobolus rotundus · Crucian carp · Carassius auratus auratus · Skin mucus · Monoclonal antibody · Diagnosis Full text in pdf format PreviousExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 54, No. 2. Online publication date: March 31, 2003 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2003 Inter-Research.