Functional Expression of MT2 (Mel1b) Melatonin Receptors in Human PAZ6 Adipocytes

褪黑素 内科学 内分泌学 褪黑激素受体 受体 脂肪组织 生物 褐色脂肪组织 脂肪细胞 过剩4 松果体 白色脂肪组织 福斯科林 葡萄糖摄取 胰岛素 医学
作者
Lena Brydon,Laurence Petit,Philippe Delagrange,A. Donny Strosberg,Ralf Jockers
出处
期刊:Endocrinology [Oxford University Press]
卷期号:142 (10): 4264-4271 被引量:166
标识
DOI:10.1210/endo.142.10.8423
摘要

Several reports have demonstrated that the pineal hormone, melatonin, plays an important role in body mass regulation in mammals. To date, however, the target tissues and relevant biochemical mechanisms involved remain uncharacterized. As adipose tissue is the principal site of energy storage in the body, we investigated whether melatonin could also act on this tissue. Semiquantitative RT-PCR analysis revealed the expression of MT1 and MT2 melatonin receptor mRNAs in the human brown adipose cell line, PAZ6, as well as in human brown and white adipose tissue. Binding analysis with 2-[125I]iodomelatonin (125I-Mel) revealed the presence of a single, high affinity binding site in PAZ6 adipocytes with a binding capacity of 7.46 ± 1.58 fmol/mg protein and a Kd of 457 ± 5 pm. Both melatonin and the MT2 receptor-selective antagonist, 4-phenyl-2-propionamidotetraline, competed with 2-[125I]iodomelatonin binding, with respective Ki values of 3 × 10−11 and 1.5 × 10−11m. Functional expression of melatonin receptors in PAZ6 adipocytes was indicated by the melatonin-induced, dose-dependent inhibition of forskolin-stimulated cAMP levels and basal cGMP levels with IC50 values of 2 × 10−9 and 3 × 10−10m, respectively. Modulation of the cGMP pathway by melatonin further supports functional expression of MT2 receptors, as this pathway was shown to be specific for that subtype in humans. In addition, long-term melatonin treatment of PAZ6 adipocytes was found to decrease the expression of the glucose transporter Glut4 and glucose uptake, an important parameter of adipocyte metabolism. These results suggest that melatonin may act directly at MT2 receptors on human brown adipocytes to regulate adipocyte physiology.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
李思发布了新的文献求助10
1秒前
liyq完成签到,获得积分10
2秒前
暮光微凉发布了新的文献求助10
2秒前
yang完成签到,获得积分10
3秒前
十一完成签到,获得积分10
3秒前
3秒前
4秒前
4秒前
lbj完成签到,获得积分20
5秒前
inferyes发布了新的文献求助10
6秒前
blawxx发布了新的文献求助10
7秒前
8秒前
上官若男应助tc采纳,获得10
8秒前
橘子完成签到,获得积分10
9秒前
Liu发布了新的文献求助150
9秒前
L2完成签到,获得积分20
9秒前
10秒前
善学以致用应助哈皮采纳,获得10
10秒前
微笑的水桃完成签到 ,获得积分10
12秒前
王才强发布了新的文献求助10
12秒前
blawxx完成签到,获得积分10
13秒前
13秒前
二十又澪完成签到,获得积分10
13秒前
Cici发布了新的文献求助10
15秒前
二十又澪发布了新的文献求助10
15秒前
16秒前
望北楼主发布了新的文献求助10
16秒前
酷波er应助Calvin采纳,获得10
17秒前
苏晓聪完成签到,获得积分10
17秒前
18秒前
奔放的老青年完成签到,获得积分10
18秒前
何止完成签到,获得积分10
19秒前
大模型应助迅速的代桃采纳,获得10
19秒前
20秒前
科研通AI2S应助1234采纳,获得10
21秒前
李二牛完成签到,获得积分10
21秒前
在水一方应助Miki采纳,获得10
21秒前
典雅碧空发布了新的文献求助10
21秒前
kimiweiwei发布了新的文献求助10
22秒前
Jc完成签到 ,获得积分10
22秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Toward a Combinatorial Approach for the Prediction of IgG Half-Life and Clearance 500
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969940
求助须知:如何正确求助?哪些是违规求助? 3514642
关于积分的说明 11175298
捐赠科研通 3249947
什么是DOI,文献DOI怎么找? 1795178
邀请新用户注册赠送积分活动 875617
科研通“疑难数据库(出版商)”最低求助积分说明 804891