Functional Expression of MT2 (Mel1b) Melatonin Receptors in Human PAZ6 Adipocytes

褪黑素 内科学 内分泌学 褪黑激素受体 受体 脂肪组织 生物 褐色脂肪组织 脂肪细胞 过剩4 松果体 白色脂肪组织 福斯科林 葡萄糖摄取 胰岛素 医学
作者
Lena Brydon,Laurence Petit,Philippe Delagrange,A. Donny Strosberg,Ralf Jockers
出处
期刊:Endocrinology [Oxford University Press]
卷期号:142 (10): 4264-4271 被引量:166
标识
DOI:10.1210/endo.142.10.8423
摘要

Several reports have demonstrated that the pineal hormone, melatonin, plays an important role in body mass regulation in mammals. To date, however, the target tissues and relevant biochemical mechanisms involved remain uncharacterized. As adipose tissue is the principal site of energy storage in the body, we investigated whether melatonin could also act on this tissue. Semiquantitative RT-PCR analysis revealed the expression of MT1 and MT2 melatonin receptor mRNAs in the human brown adipose cell line, PAZ6, as well as in human brown and white adipose tissue. Binding analysis with 2-[125I]iodomelatonin (125I-Mel) revealed the presence of a single, high affinity binding site in PAZ6 adipocytes with a binding capacity of 7.46 ± 1.58 fmol/mg protein and a Kd of 457 ± 5 pm. Both melatonin and the MT2 receptor-selective antagonist, 4-phenyl-2-propionamidotetraline, competed with 2-[125I]iodomelatonin binding, with respective Ki values of 3 × 10−11 and 1.5 × 10−11m. Functional expression of melatonin receptors in PAZ6 adipocytes was indicated by the melatonin-induced, dose-dependent inhibition of forskolin-stimulated cAMP levels and basal cGMP levels with IC50 values of 2 × 10−9 and 3 × 10−10m, respectively. Modulation of the cGMP pathway by melatonin further supports functional expression of MT2 receptors, as this pathway was shown to be specific for that subtype in humans. In addition, long-term melatonin treatment of PAZ6 adipocytes was found to decrease the expression of the glucose transporter Glut4 and glucose uptake, an important parameter of adipocyte metabolism. These results suggest that melatonin may act directly at MT2 receptors on human brown adipocytes to regulate adipocyte physiology.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
Singularity应助内向寒云采纳,获得10
1秒前
dd完成签到 ,获得积分10
1秒前
2秒前
Miracle发布了新的文献求助10
3秒前
开花完成签到,获得积分10
3秒前
量子星尘发布了新的文献求助10
4秒前
5秒前
redking完成签到,获得积分10
5秒前
lingquanmeng完成签到,获得积分10
6秒前
6秒前
羽生发布了新的文献求助10
6秒前
隐形曼青应助miumiu采纳,获得10
7秒前
beckham发布了新的文献求助10
8秒前
施天问完成签到,获得积分10
9秒前
uu发布了新的文献求助10
9秒前
10秒前
10秒前
10秒前
小蘑菇应助Miracle采纳,获得10
10秒前
轩轩轩轩完成签到 ,获得积分10
11秒前
11秒前
12秒前
litao完成签到,获得积分10
13秒前
乐乐应助九千七采纳,获得10
15秒前
梁三柏应助makabaka采纳,获得10
15秒前
16秒前
16秒前
xyz发布了新的文献求助10
17秒前
20秒前
tree完成签到,获得积分10
20秒前
miumiu完成签到,获得积分20
20秒前
xiaoyu1完成签到,获得积分10
21秒前
小鞋发布了新的文献求助10
22秒前
鳗鱼白竹完成签到,获得积分10
23秒前
25秒前
Chen完成签到 ,获得积分10
26秒前
beckham发布了新的文献求助10
27秒前
wangxiaoyating完成签到,获得积分10
27秒前
宋嘉新完成签到,获得积分10
27秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975755
求助须知:如何正确求助?哪些是违规求助? 3520108
关于积分的说明 11200829
捐赠科研通 3256492
什么是DOI,文献DOI怎么找? 1798298
邀请新用户注册赠送积分活动 877509
科研通“疑难数据库(出版商)”最低求助积分说明 806403