Next generation sequencing reveals microRNA isoforms in liver cirrhosis and hepatocellular carcinoma

肝细胞癌 生物 癌症研究 癌症 癌变 内科学 转录组 基因
作者
Anna Wojcicka,Michal Swierniak,Oskar Kornasiewicz,Wojciech Gierlikowski,Monika Maciag,Monika Kolanowska,Marta Kotlarek,Barbara Górnicka,Lukasz Koperski,Grzegorz Niewiński,Marek Krawczyk,Krystian Jazdzewski
出处
期刊:The International Journal of Biochemistry & Cell Biology [Elsevier BV]
卷期号:53: 208-217 被引量:71
标识
DOI:10.1016/j.biocel.2014.05.020
摘要

a b s t r a c t Hepatocellular carcinoma (HCC) represents the major histological subtype of liver cancer. Tumorigenic changes in hepatic cells potentially result from aberrant expression of microRNAs (miRNAs). Individual microRNA gene may give rise to miRNAs of different length, named isomiRNAs that proved to be func- tionally relevant. Since microRNA length heterogeneity in hepatic tissue has not been described before, we employed next-generation sequencing to comprehensively analyze microRNA transcriptome in HCC tumors (n = 24) and unaffected tissue adjacent to tumors (n = 24), including samples with (n = 15) and without cirrhosis (n = 9). We detected 374 microRNAs expressed in liver, including miR-122-5p that constituted over 39% of the hepatic miRnome. Among the liver expressed miRs, the levels of 64 significantly differed between tumor and control samples (FDR 2). Top deregulated miRNAs included miR-1269a (T/N = 22.95), miR-3144-3p (T/N = 5.24), miR-183-5p (T/N = 4.63), miR-10b-5p (T/N = 3.87), miR-490-3p (T/N = 0.13), miR-199a-5p (T/N = 0.17), miR-199a-3p/miR-199b-3p (T/N = 0.19), miR-214-5p (T/N = 0.20) and miR-214-3p (T/N = 0.21). Almost all miRNA genes produced several mature molecules differing in length (isomiRNAs). The reference sequence was not the most prevalent in 38.6% and completely absent in 10.5% of isomiRNAs. Over 26.1% of miRNAs produced isoforms carrying ≥ 2 alternative seed regions, of which 35.5% constituted novel, previously unknown seeds. This fact sheds new light on the percentage of the human genome regulated by microRNAs and their variants. Among the most deregulated miR- NAs, miR-199a-3p/miR-199b-3p (T/N fold change = 0.18, FDR = 0.005) was expressed in 9 isoforms with 3 different seeds, concertedly leading to upregulation of TGF-beta signaling pathway (OR = 1.99; p = 0.004). In conclusion, the study reveals the comprehensive miRNome of hepatic tissue and provides new tools for investigation of microRNA-dependent pathways in cirrhotic liver and hepatocellular carcinoma. This article is part of a Directed Issue entitled: Rare Cancers.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
xiangrikui完成签到,获得积分0
刚刚
江岸与城完成签到 ,获得积分10
1秒前
2秒前
CodeCraft应助董啊采纳,获得10
2秒前
魔力巴啦啦完成签到 ,获得积分10
3秒前
自信雅琴发布了新的文献求助20
3秒前
3秒前
许鑫蓁完成签到 ,获得积分10
3秒前
lulu加油完成签到,获得积分10
4秒前
4秒前
xiangrikui发布了新的文献求助10
4秒前
牛马完成签到 ,获得积分10
5秒前
科研通AI5应助WJH采纳,获得10
6秒前
Zard发布了新的文献求助10
6秒前
王冉冉完成签到,获得积分10
7秒前
ryan1300完成签到 ,获得积分10
7秒前
易拉罐完成签到,获得积分10
7秒前
ZQ完成签到,获得积分10
7秒前
yyds完成签到,获得积分20
7秒前
7秒前
8秒前
彭于晏应助刘宇采纳,获得10
8秒前
9秒前
leeom发布了新的文献求助10
11秒前
Timo干物类完成签到,获得积分10
11秒前
北冥有鱼给北冥有鱼的求助进行了留言
11秒前
11秒前
王冉冉发布了新的文献求助30
11秒前
Ava应助易拉罐采纳,获得10
12秒前
隐形曼青应助无心的土豆采纳,获得10
12秒前
乐于助人大好人完成签到 ,获得积分10
12秒前
ZZQ完成签到,获得积分10
16秒前
量子星尘发布了新的文献求助10
16秒前
Lina HE完成签到 ,获得积分10
16秒前
852应助科研通管家采纳,获得10
16秒前
Ava应助科研通管家采纳,获得10
17秒前
ED应助科研通管家采纳,获得10
17秒前
领导范儿应助科研通管家采纳,获得10
17秒前
17秒前
Akim应助科研通管家采纳,获得10
17秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038569
求助须知:如何正确求助?哪些是违规求助? 3576279
关于积分的说明 11374944
捐赠科研通 3305979
什么是DOI,文献DOI怎么找? 1819354
邀请新用户注册赠送积分活动 892698
科研通“疑难数据库(出版商)”最低求助积分说明 815048