长春新碱
细胞毒性
胶质瘤
细胞培养
诺可达唑
医学
体外
细胞
细胞生长
球体
苯妥英钠
溴脱氧尿苷
流式细胞术
庆大霉素保护试验
癌症研究
病理
细胞生物学
生物
免疫学
内科学
生物化学
化疗
细胞骨架
环磷酰胺
免疫组织化学
癌症
转移
癫痫
精神科
遗传学
作者
Jörg‐Christian Tonn,Hans Kristian Haugland,Jaakko Saraste,K. Roosen,Ole Didrik Lærum
出处
期刊:Journal of Neurosurgery
[Journal of Neurosurgery Publishing Group]
日期:1995-06-01
卷期号:82 (6): 1035-1043
被引量:23
标识
DOI:10.3171/jns.1995.82.6.1035
摘要
✓ The aim of this study was to investigate the antimigratory and antiinvasive potential of vincristine sulfate (VCR) on human glioma cells and to analyze whether phenytoin (5,5-diphenylhydantoin; DPH) might act synergistically with VCR. Vincristine affects the cytoplasmic microtubules; DPH has been reported to enhance VCR cytotoxicity in murine cells. In two human glioma cell lines, GaMG and D-37MG, we found VCR to reduce monolayer growth and colony formation in a dose-dependent fashion at concentrations of 10 ng/ml and above. Phenytoin increased the cytotoxic and cystostatic effects of VCR in monolayer cells but not in spheroids. Multicellular spheroids were used to investigate directional migration. A coculture system of GaMG and D-37MG spheroids with fetal rat brain aggregates was used to analyze and quantify tumor cell invasion. A dose-dependent inhibition of migration and invasion by VCR was observed in both cell lines without further enhancement by DPH. Immunofluorescence microscopy with antibodies against α-tubulin revealed dose-dependent morphological alterations in the microtubules when the cells were exposed to VCR but not after incubation with DPH. Based on the combination of standardized in vitro model systems currently in use and the present data, the authors strongly suggest that VCR inhibits migration and invasion of human glioma cells. This is not altered by DPH, which inhibits cell proliferation in combination with VCR.
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