Screening of SIRT5 inhibitors from natural products

Objective: To screen inhibitors for the potential tumor therapeutic target of SIRT5( sirtuin5)from natural products. Methods: An HPLC-based method was established for detecting the desuccinylase activity of SIRT5. SIRT5 inhibitors were screened from natural products and their IC50 values were then measured. Results:The IC50 sof oleanolic acid and echinocystic acid which contained a carboxyl group were 40 和 70 μmol·L-1,respectively. Conclusion: The natural products such as oleanolic acid and echinocystic acid can obviously inhibit the desuccinylase activity of SIRT5. This finding will facilitate further study on the medicinal chemistry targeting SIRT5.