化学
阿布茨
抗氧化剂
TBARS公司
羟基自由基
DPPH
木瓜蛋白酶
抗坏血酸
硫代巴比妥酸
螯合作用
超氧化物
水解
食品科学
有机化学
色谱法
核化学
脂质过氧化
酶
作者
Balasubramanian Sivaraman,R. Jeya Shakila,G. Jeyasekaran,D. Sukumar,Uthaman Manimaran,G. Sumathi
标识
DOI:10.1007/s10068-016-0117-4
摘要
Squid protein hydrolysates (SPH) were prepared from the Indian squid Loligo duvauceli using papain. Response surface methodology (RSM) was used for optimization of hydrolysis conditions, including temperature, time, and the enzyme-substrate ratio using DPPH radical scavenging activity as a response. The amino acid composition of SPH was compared with raw squid muscle. In vitro antioxidant activities were evaluated based on reducing power, metal chelation, ABTS, hydroxyl radical, and superoxide anion radical scavenging assays. SPH exhibited good ABTS radical scavenging activities of 96.50±0.90%, superoxide anion radical scavenging activities of 96.4±0.89%, reducing powers of 0.71±0.02, moderate hydroxyl radical scavenging activities of 64.03±2.11%, and metal chelating activities of 52.04±1.02%. In vivo antioxidant activities determined using a sardine minced model system showed 42% reduction in formation of secondary oxidative products as thiobarbituric acid reactive substances (TBARS), almost equivalent to reduction by ascorbic acid of 41.42% at 400 ppm.
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