谷胱甘肽
谷胱甘肽还原酶
谷胱甘肽二硫化物
硫醇
化学
谷胱甘肽
吸光度
GPX4
生物化学
还原酶
二硫键
激进的
酶
色谱法
谷胱甘肽过氧化物酶
标识
DOI:10.1002/0471140856.tx0702s00
摘要
Abstract Reduced glutathione, a thiol, is essential to the survival of the cells of most aerobic organisms. It is present intracellularly and provides protection from hydroperoxides and free radicals. This unit describes a continuous spectrophotometric assay for reductase activity: it follows the reduction of glutathione disulfide to reduced glutathione by monitoring the oxidation of NADPH as visualized by a decrease in absorbance at 340 nm.
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