Molecular cloning on small subunit SSU I gene of AGPase in wheat and construction of its overexpression,antisense and RNAi expression vectors

Small subsuit gene I of AGPase (SSU I) full cDNA sequences was cloned and its sense,antisense and RNAi vectors were constructed in this paper.SSU I gene cDNA sequences (1 465 bp) (GenBank No. EF405961) from grains of common wheat(Triticum aestivum L.,Yujiao 2 cultivar) were amplified through RT-PCR and the coding sequences of this gene were analyzed.The result demonstrated that the coding region of cloned SSU I was 1 422 bp (2～1423 bp in EF405961),which shared 98%～99% high homogeneity to sequences of X66080,AF244997,Z48562 genes.In order to study the function of SSU I gene,its sense expression vector was constructed,in which the coding region of the gene was placed under the 35S promoter of pWM101 in sense orientation.And antisense expression vector was also constructed with pBI121 plasmids. In addition,260 bp eDNA fragments of SSU I was also cloned,and RNAi expression vector of this gene was constructed with pFGC5941 vector.