表面等离子共振
生物传感器
化学
胶体金
检出限
纳米技术
干扰(通信)
等离子体子
吸附
小RNA
纳米颗粒
频道(广播)
共振(粒子物理)
光电子学
材料科学
色谱法
计算机科学
物理
电信
基因
有机化学
粒子物理学
生物化学
作者
Ming‐Wei Lin,Chen Wang,Ruizhi Fan,Xinya Zhao,Li Yu,Mengdi Lu,Wei Peng
出处
期刊:Talanta
[Elsevier]
日期:2024-04-23
卷期号:275: 126142-126142
被引量:4
标识
DOI:10.1016/j.talanta.2024.126142
摘要
A multi-channel prismatic localized surface plasmon resonance (LSPR) biosensor was developed for quantitative and real-time detection of multiple COVID-19 characteristic miRNAs. The well-dispersed and dense gold nanoparticles (AuNPs) arrays for LSPR biosensing were fabricated through a nano-thickness diblock copolymer template (BCPT). Both theoretical and experimental analyses were conducted to investigate the effects of particle size, interparticle spacing, and surface coverage on LSPR sensing spectrum and intensity sensitivity of varied AuNPs sizes. A competitive assay strategy was proposed and used for non-amplification miRNA detection with a low limit detection of 3.41 nM, while a four-channel prismatic LSPR system enables parallel detection of multiple miRNAs. Furthermore, this sensing strategy can effectively and specifically identify target miRNA, distinguish mismatched miRNA and interfering miRNA, and exhibit low non-specific adsorption. This BCPT-based LSPR biosensor demonstrates the practicality and potential of a multi-channel, adaptable, and integrated prismatic sensor in medical testing and diagnostic applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI