免疫系统
信号转导
生物
细胞生物学
微生物学
免疫学
作者
Hua Yu,Weiying Xu,Sisi Chen,Xiaoxian Wu,Weiwei Rao,Lei Zhu,Xiaoyan Xu,Jingqi Chen,Marc T. Nishimura,Yu Zhang,Li Wan
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2024-11-07
标识
DOI:10.1126/science.adr3150
摘要
Plant intracellular nucleotide-binding leucine-rich repeat (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain sense pathogen effectors to initiate immune signaling. TIR domains across different kingdoms possess NADase activities and can produce phosphoribosyl adenosine monophosphate/diphosphate (pRib-AMP/ADP) or cyclic ADPR (cADPR) isomers. Lipase-like proteins, EDS1 and PAD4, transduce immune signals from sensor TIR-NLRs to a helper NLR called ADR1, which executes immune function. We report the structure and function of Arabidopsis EDS1-PAD4-ADR1 (EPA) heterotrimer in complex with pRib-AMP/ADP activated by plant or bacterial TIR signaling. 2’cADPR can be hydrolyzed into pRib-AMP and thus activates EPA signaling. Bacterial TIR domains producing 2’cADPR also activate EPA function. Our findings suggest that 2’cADPR may be the storage form of the unstable signaling molecule pRib-AMP.
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