Effect of transglutaminase crosslinking on the protein structure and potential allergenicity of dual-protein system, with a focus on β-conglycinin

组织谷氨酰胺转胺酶 化学 大豆蛋白 乳清蛋白 分离乳清蛋白粉 抗原性 过敏原 蛋白质聚集 生物化学 生物物理学 食品科学 抗原 生物 过敏 免疫学
作者
Yifan Shi,Ting Tang,Tianran Hui,Yuying Chang,Xiyun Chen,Guangliang Xing
出处
期刊:Lebensmittel-Wissenschaft & Technologie [Elsevier BV]
卷期号:188: 115417-115417 被引量:7
标识
DOI:10.1016/j.lwt.2023.115417
摘要

Transglutaminase (TGase)-mediated crosslinking serves as a promising strategy to mitigate soy antigenicity. β-Conglycinin is recognized as a key allergen among soy proteins. Nonetheless, our understanding of the underlying relationship between immunoreactivity and protein structure remains inadequate. This study aimed to examine the impact of TGase-mediated crosslinking on the immunoreactivity of β-conglycinin in a dual-protein system consisting of soy protein isolate (SPI) and whey protein isolate (WPI) (with SPI-WPI ratios of 10:0, 7:3, 5:5, 3:7 (w/w)). Conformational changes were also examined. Results revealed that β-lactoglobulin and α-lactalbumin in WPI were less susceptible to TGase crosslinking compared to the 7S and 11S subunits in SPI. The conformation of proteins in the dual-protein system after TGase crosslinking were altered, as demonstrated by increased ultraviolet absorption intensity, fluctuant intrinsic fluorescence intensity and surface hydrophobicity, and decreased free sulfhydryl content. As the crosslinking time of TGase increased from 1 h to 18 h, the allergenicity of β-conglycinin gradually decreased in all four SPI-WPI ratios. Notably, the SPI3-WPI7 ratio exhibited the lowest allergenicity of 77.21 ± 1.89% after 18 h of TGase catalyzation. Overall, the dual-protein system proved to be more effective in reducing the allergenicity of β-conglycinin.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
乐观小蕊发布了新的文献求助10
刚刚
量子星尘发布了新的文献求助10
刚刚
lhy完成签到,获得积分10
1秒前
小马甲应助yetong采纳,获得10
4秒前
丘比特应助友好的听寒采纳,获得10
6秒前
6秒前
乐观小蕊完成签到,获得积分10
8秒前
果果完成签到,获得积分10
10秒前
温柔的老头完成签到,获得积分10
17秒前
18秒前
完美世界应助猪猪hero采纳,获得10
18秒前
18秒前
19秒前
iNk应助欧阳采纳,获得10
19秒前
23秒前
晚风发布了新的文献求助10
23秒前
Junex发布了新的文献求助10
24秒前
乐观幻天完成签到,获得积分10
24秒前
斯文败类应助科研通管家采纳,获得30
26秒前
Lucas应助科研通管家采纳,获得10
26秒前
26秒前
元万天应助黒子采纳,获得50
26秒前
26秒前
隐形曼青应助科研通管家采纳,获得10
26秒前
26秒前
26秒前
思源应助科研通管家采纳,获得10
26秒前
26秒前
27秒前
27秒前
28秒前
8R60d8应助谦让之云采纳,获得10
29秒前
请叫我风吹麦浪应助哈哈采纳,获得10
31秒前
柔弱紊发布了新的文献求助10
32秒前
猪猪hero发布了新的文献求助10
33秒前
万能图书馆应助柔弱紊采纳,获得10
38秒前
L912294993完成签到,获得积分10
39秒前
40秒前
42秒前
量子星尘发布了新的文献求助10
44秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3971606
求助须知:如何正确求助?哪些是违规求助? 3516269
关于积分的说明 11181779
捐赠科研通 3251428
什么是DOI,文献DOI怎么找? 1795887
邀请新用户注册赠送积分活动 876110
科研通“疑难数据库(出版商)”最低求助积分说明 805246