Freezing-assisted sugaring-out liquid-liquid extraction coupled with LC-MS/MS for quantitative determination of perchlorate in honey

For the first time, a simple, quick, sensitive, and low cost method for quantification of perchlorate in honey using liquid chromatography-tandem mass spectrometry was developed. Through freezing-assisted sugaring-out liquid-liquid extraction, one-step simultaneous extraction and clean-up of perchlorate from honey were perfectly achieved. Glucose and fructose, the most abundant sugars in honey, were almost completely removed from the extract without use of any clean-up materials. Under optimum conditions, the proposed approach exhibited satisfactory linearity, negligible matrix effects, and low detection limit of 0.05 µg/kg, providing recoveries of 96.7 %-102.3 % with relative standard deviation of < 9 % for honey samples. The validated method was applied to the analysis of perchlorate in 36 honey samples, and detection rate was 94.4 %. This work provided a simple and reliable method for extensive monitoring of perchlorate in honey and opened- up new insights for analysis of contaminants in honey matrixes.