Serabelisib regulates GSDMD-mediated pyroptosis, apoptosis and migration of hepatoma cells via the PI3K/Akt/E-cadherin signaling pathway

PI3K/AKT/mTOR通路 蛋白激酶B 活力测定 细胞凋亡 细胞周期 细胞生长 癌症研究 上皮-间质转换 生物 细胞生物学 信号转导 化学 分子生物学 癌症 转移 生物化学 遗传学
作者
Ming Li,Zhao Huang,Yuxiang Zhou
出处
期刊:Advances in Clinical and Experimental Medicine [Wroclaw Medical University]
卷期号:33 (2): 171-181 被引量:1
标识
DOI:10.17219/acem/166511
摘要

Background.Liver cancer is a malignant tumor commonly seen in infants and young children.Serabelisib is a novel and effective phosphoinositide-3-kinase (PI3Kα) inhibitor, currently in trials for solid malignancy treatment, such as bladder cancer.However, it is unclear whether serabelisib affects liver cancer. Objectives.To explore the effects of serabelisib on the proliferation, apoptosis, invasion, and metastasis of HepG2 and HuH-6 cells, and elucidate the relevant molecular mechanisms.Materials and methods.The HepG2 cells were treated with 2 μM, 4 μM or 8 μM serabelisib, while the 0-μM group was used as a control.A plate clone formation assay was utilized to measure colony formation ability in each group, a 5-ethynyl-2'-deoxyuridine (EdU) assay examined cell proliferation, a Cell Counting Kit-8 (CCK-8) assay assessed cell viability, flow cytometry measured the cell cycle and apoptosis, JC-1 staining determined mitochondrial membrane potential, and transmission electron microscopy evaluated cell morphology.In addition, gene and protein expression levels of apoptosis markers, epithelial-mesenchymal transition (EMT), Gasdermin D (GSDMD), and the PI3K/protein kinase B (AKT) signaling pathway were measured.Results.After serabelisib intervention, HepG2 and HuH-6 cells formed fewer colonies, proliferated more slowly, and had reduced viability.The number of HepG2 and HuH-6 cells in the G2 and S phases decreased, apoptosis and the number of apoptotic bodies increased, and mitochondrial membrane potential decreased.Serabelisib treatment also reduced the migration and invasion capacity of the cells.Furthermore, genes and proteins of the PI3K/AKT signaling pathway were downregulated, while those that promote apoptosis and pyroptosis or inhibit EMT were upregulated.Conclusions.Serabelisib inhibited the PI3K/AKT signaling pathway, thereby inhibiting EMT and promoting apoptosis and pyroptosis in HepG2 and HuH-6 cells.
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