清脆的
换位(逻辑)
生物
遗传学
基因
DNA
流动遗传元素
插入顺序
基因组
核酸酶
大肠杆菌
计算生物学
基因组DNA
Cas9
转座因子
哲学
语言学
作者
Yong Sheng,Hengyu Wang,Yixin Ou,Ying‐Ying Wu,Wei Ding,Meifeng Tao,Shuangjun Lin,Zixin Deng,Linquan Bai,Qianjin Kang
标识
DOI:10.1038/s41467-023-39964-7
摘要
CRISPR-Cas immunity systems safeguard prokaryotic genomes by inhibiting the invasion of mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified multiple natural transpositions of insertion sequences (ISs) into cas genes, thus inactivating CRISPR-Cas defenses. We then generated an IS-trapping system, using Escherichia coli strains with various ISs and an inducible cas nuclease, to monitor IS insertions into cas genes following the induction of double-strand DNA breakage as a physiological host stress. We identified multiple events mediated by different ISs, especially IS1 and IS10, displaying substantial relaxed target specificity. IS transposition into cas was maintained in the presence of DNA repair machinery, and transposition into other host defense systems was also detected. Our findings highlight the potential of ISs to counter CRISPR activity, thus increasing bacterial susceptibility to foreign DNA invasion.
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