生物发光
荧光素酶
细菌
生物发光成像
化学
荧光素
生化工程
纳米技术
生物化学
生物
工程类
材料科学
转染
遗传学
基因
作者
Shitong Liu,Jinbin Zhao,Yulan Guo,Xueer Ma,Jiasheng Tu,Ming Cai,Y.M. Chi,Kun Xu
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:148 (15): 3452-3459
被引量:7
摘要
With the development of new technologies for rapid and high-throughput bacterial detection, ATP-based bioluminescence technology is making progress. Because live bacteria contain ATP, the number of bacteria is correlated with the level of ATP under certain conditions, so that the method of luciferase catalyzing the fluorescence reaction of luciferin with ATP is widely used for the detection of bacteria. This method is easy to operate, has a short detection cycle, does not require much human resources, and is suitable for long-term continuous monitoring. Currently, other methods are being explored in combination with bioluminescence for more accurate, portable and efficient detection. This paper introduces the principle, development and application of bacterial bioluminescence detection based on ATP and compares the combination of bioluminescence and other bacterial detection methods in recent years. In addition, this paper also examines the development prospects and direction of bioluminescence in bacterial detection, hoping to provide a new idea for the application of ATP-based bioluminescence.
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