Biofunctional Inorganic Layered Double Hydroxide Nanohybrid Enhances Immunotherapeutic Effect on Atopic Dermatitis Treatment

Abstract Atopic dermatitis (AD) is a widespread, recurrent, and chronic inflammatory skin condition that imposes a major burden on patients. Conventional treatments, such as corticosteroids, are associated with various side effects, underscoring the need for innovative therapeutic approaches. In this study, the possibility of using indole‐3‐acetic acid‐loaded layered double hydroxides (IAA‐LDHs) is evaluated as a novel treatment for AD. IAA is an auxin‐class plant hormone with antioxidant and anti‐inflammatory effects. Following the synthesis of IAA‐LDH nanohybrids, their ability to induce M2‐like macrophage polarization in macrophages obtained from mouse bone marrow is assessed. The antioxidant activity of IAA‐LDH is quantified by assessing the decrease in intracellular reactive oxygen species levels. The anti‐inflammatory and anti‐atopic characteristics of IAA‐LDH are evaluated in a mouse model of AD by examining the cutaneous tissues, immunological organs, and cells. The findings suggest that IAA‐LDH has great therapeutic potential as a candidate for AD treatment based on its in vitro and in vivo modulation of AD immunology, enhancement of macrophage polarization, and antioxidant activity. This inorganic drug delivery technology represents a promising new avenue for the development of safe and effective AD treatments.