内部收益率3
生物
病毒学
免疫系统
干扰素
基因
坦克结合激酶1
分子生物学
信号转导
癌症研究
先天免疫系统
细胞生物学
免疫学
遗传学
丝裂原活化蛋白激酶激酶
蛋白激酶C
作者
Quanfan Chen,Xiao-an WANG,S.W. Jiang,Pan Zhang,S.Y. Huang,Yongjun LIANG,Hanbo Jia,H.F. Zhu
出处
期刊:Polish Journal of Veterinary Sciences
[De Gruyter]
日期:2023-03-06
卷期号:: 119-130
被引量:7
标识
DOI:10.24425/pjvs.2023.145013
摘要
African swine fever virus (ASFV) causes feverous and hemorrhagic disease of domestic pigs and European wild boars with high mortality, yet no commercial vaccine is currently available. Several ASFV strains with natural deletion or gene-targeted knockout of multiple MGF360 and MGF505 genes are attenuated in vitro and in vivo, and can offer full protection against homologous challenge. However, the mechanisms underlying the protection are not fully understood. This study aims to investigate the effects of MGF360-12L of ASFV-SY18 on the cGAS-STING signaling pathway and explore the potential mechanisms. We identified that ASFV-SY18 MGF360-12L could inhibit cGAS-STING, TBK1, or IRF3-5D-stimulated IFN-β expression and ISRE activation. Specifically, MGF360-12L inhibits both the activation of PRD(III-I) in a dose-dependent manner, and suppresses the exogenous expression of TBK1 and IRF3-5D. MGF360-12L could block NF-κB activation induced by overexpression of cGAS-STING, TBK1, IKKβ. Downstream of the IFN-β signaling, MGF360-12L blocks the ISRE promoter activation by reducing total protein level of IRF9. Moreover, MGF360-12L protein can inhibit IFN-β-mediated antiviral effects. In conclusion, our findings suggest that MGF360-12L is a multifunctional immune-evasion protein that inhibits both the expression and effect of IFN-β, which could partially explain the attenuation of relevant gene-deleted ASFV strains, and shed light on the development of efficient ASFV live attenuated vaccines in the future.
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